BN52021对重症急性胰腺炎大鼠胰腺组织中核因子κB p65表达的影响
Effect of BN52021 on NFkappa-Bp65 expression in pancreatic tissues of rats with severe acute pancreatitis.
作者信息
Xia Shi-Hai, Fang Dian-Chun, Hu Chun-Xiu, Bi Hui-Ying, Yang Yin-Zhi, Di Yao
机构信息
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
出版信息
World J Gastroenterol. 2007 Feb 14;13(6):882-8. doi: 10.3748/wjg.v13.i6.882.
AIM
To investigate dynamic changes and significance of expression of NF-kappaBp65 in pancreatic tissues of rats with severe acute pancreatitis (SAP), as well as BN52021 effects.
METHODS
Wistar male rats were randomly divided into negative control group (NC group, n=60), SAP-model group (SAP group, n=60), and BN52021-treated group (BN group, n=60), and each of the above groups was respectively divided into 6 subgroups at different time points after operation (1 h, 2 h, 3 h, 6 h, 12 h, and 24 h) (n=10). By RT-PCR and Western blot, NF-kappaBp65 mRNA and its protein expression in pancreatic tissues of rats were detected respectively.
RESULTS
The expression of NF-kappaBp65 mRNA dynamically changed in both SAP groups and BN groups. The mRNA level was higher in SAP groups than NC groups at 2 h, 3 h, 12 h, and 24 h after operation (P<0.05), higher in BN groups than NC groups at all time points (P<0.05), and higher in BN groups than SAP group at 1 h (P<0.05). The NF-kappaBp65 protein level was higher in SAP groups than NC groups at 1 h, 3 h, and 6 h (P<0.01), and 2 h, 12 h, and 24 h (P<0.05), higher in BN groups than NC groups at all time points (P<0.05), and lower in BN groups than SAP groups at 1 h, 3 h, and 6 h (P<0.05).
CONCLUSION
The expression of NF-kappaBp65 in pancreatic tissues is dynamically changed and the changes play an important role in pathogenesis of SAP. BN52021 exerts therapeutic effects through reducing the expression level of NF-kappaBp65 protein in the early stage of SAP.
目的
探讨核因子-κB p65（NF-κB p65）在重症急性胰腺炎（SAP）大鼠胰腺组织中的表达动态变化及意义，以及BN52021的作用。
方法
将雄性Wistar大鼠随机分为阴性对照组（NC组，n = 60）、SAP模型组（SAP组，n = 60）和BN52021治疗组（BN组，n = 60），上述每组在术后不同时间点（1 h、2 h、3 h、6 h、12 h和24 h）再分别分为6个亚组（n = 10）。采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western blot）分别检测大鼠胰腺组织中NF-κB p65 mRNA及其蛋白表达。
结果
NF-κB p65 mRNA在SAP组和BN组中均呈动态变化。术后2 h、3 h、12 h和24 h，SAP组mRNA水平高于NC组（P < 0.05）；各时间点BN组均高于NC组（P < 0.05）；术后1 h，BN组高于SAP组（P < 0.05）。NF-κB p65蛋白水平在术后1 h、3 h和6 h，SAP组高于NC组（P < 0.01），2 h、12 h和24 h，SAP组高于NC组（P < 0.05）；各时间点BN组均高于NC组（P < 0.05）；术后1 h、3 h和6 h BN组低于SAP组（P < 0.05）。
结论
胰腺组织中NF-κB p65表达呈动态变化，且该变化在SAP发病机制中起重要作用。BN52021通过降低SAP早期NF-κB p65蛋白表达水平发挥治疗作用。
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