Chen Kuan-Hao, Mukaisho Ken-Ichi, Ling Zhi-Qiang, Shimomura Akihiko, Sugihara Hiroyuki, Hattori Takanori
Department of Pathology, Shiga University of Medical Science, Seta-tsukinowa-cho, Ohtsu, Shiga 520-2192, Japan.
Anticancer Res. 2007 Jan-Feb;27(1A):175-81.
Squamous cell carcinoma (SCC) of the human esophagus has a multifactorial etiology involving several environmental and/or genetic factors. Recently, gastroesophageal reflux has been implicated as a causative factor in upper aerodigestive tract carcinogenesis. The development of esophageal squamous cell carcinoma (ESCC) in a duodenal contents reflux model without any known carcinogen present has been reported previously. In this study, the duodenal contents reflux model without gastrectomy was used. At 60 weeks post-operatively, all surviving animals had malignant lesions as follows: ESCC (40%), esophageal adenocarcinoma (EAC) (20%) and adenosquamous carcinoma (40%). In one subject, a well-differentiated ESCC was detected with thoracic dissemination and metastases in lymph nodes. A novel cell line, designated ESCC-DR, was established from the thoracic metastatic tumor at the 60th post-operative week. These cells were transplanted into nude mice, and the developed nodules represented a well differentiated ESCC, resembling that of the parent site. Duodenal contents reflux has a great potential for malignant initiation and plays a role in developing not only EAC but also ESCC.
人类食管鳞状细胞癌（SCC）具有多因素病因，涉及多种环境和/或遗传因素。最近，胃食管反流被认为是上消化道呼吸道致癌的一个致病因素。先前已有报道在不存在任何已知致癌物的十二指肠内容物反流模型中发生食管鳞状细胞癌（ESCC）。在本研究中，使用了未行胃切除术的十二指肠内容物反流模型。术后60周，所有存活动物均出现恶性病变，如下：ESCC（40%）、食管腺癌（EAC）（20%）和腺鳞癌（40%）。在一个病例中，检测到一例高分化ESCC伴有胸段播散和淋巴结转移。在术后第60周从胸段转移瘤建立了一种新的细胞系，命名为ESCC-DR。将这些细胞移植到裸鼠体内，形成的结节表现为高分化ESCC，与原发部位相似。十二指肠内容物反流具有很大的恶性起始潜能，不仅在EAC的发生中起作用，而且在ESCC的发生中也起作用。
