Kodrík Dalibor, Krishnan Natraj, Habustová Oxana
Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branisovská 31, Ceské Budejovice, Czech Republic.
Peptides. 2007 May;28(5):974-80. doi: 10.1016/j.peptides.2007.01.017. Epub 2007 Feb 6.
The level of adipokinetic hormones (AKHs) (Peram-CAH-I and II) in the corpora cardiaca and the hemolymph of Leptinotarsa decemlineata enormously increases in the adults fed on genetically modified potatoes containing either GNA lectin or Cry 3Aa toxin concomitant with increased oxidative stress in gut tissues. A similar enhancement of the AKH titer is achieved when the adults are injected with paraquat that evokes oxidative stress. On the other hand, an injection of exogenous AKH reduces oxidative stress biomarkers in the hemolymph by reducing protein carbonyls and enhancing reduced glutathione levels. These facts indicate that there is a feedback regulation between an oxidative stressor action and the level of AKH in the insect body, and that AKHs might be involved in the activation of an antioxidant protection mechanism. These results are to our knowledge, the first evidence for the involvement of AKHs in oxidative stress mitigation, in addition to a plethora of other roles.
以含有雪花莲凝集素（GNA）或Cry 3Aa毒素的转基因马铃薯为食的成年马铃薯甲虫（Leptinotarsa decemlineata），其心侧体和血淋巴中的脂肪动激素（AKHs）（Peram-CAH-I和II）水平大幅增加，同时肠道组织中的氧化应激也增强。当给成虫注射可引发氧化应激的百草枯时，也会出现类似的AKH滴度升高情况。另一方面，注射外源性AKH可通过减少蛋白质羰基化并提高还原型谷胱甘肽水平，降低血淋巴中的氧化应激生物标志物水平。这些事实表明，昆虫体内氧化应激源作用与AKH水平之间存在反馈调节，且AKHs可能参与抗氧化保护机制的激活。据我们所知，这些结果首次证明了AKHs除了具有众多其他作用外，还参与氧化应激缓解。
