Zhou Yi Jun, Fukumura Keisuke, Nagata Shinji
a Department of Integrated Biosciences, Graduate School of Frontier Sciences , The University of Tokyo , Chiba , Japan.
Biosci Biotechnol Biochem. 2018 Feb;82(2):274-284. doi: 10.1080/09168451.2017.1422106. Epub 2018 Jan 12.
Adipokinetic hormone (AKH) regulates energy homeostasis in insects by mobilizing lipid and carbohydrate from the fat body. Here, using RNA sequencing data, we identified cDNAs encoding AKH (GbAKH) and its highly homologous hormone AKH/corazonin-related peptide (GbACP) in the corpora cardiaca of the two-spotted cricket, Gryllus bimaculatus. RT-PCR revealed that GbAKH and GbACP are predominantly expressed in the corpora cardiaca and corpora allata, respectively. Phylogenetic analysis confirmed that the identified GbAKH and GbACP belong to the clades containing other AKHs and ACPs, respectively. Injection of synthetic GbAKH and GbACP elevated hemolymph carbohydrate and lipid levels and reduced food intake significantly. In contrast, knockdown of GbAKH and GbACP by RNA interference increased the food intake, although hemolymph lipid level was not altered. Collectively, this study provides evidence that ACP regulates hemolymph carbohydrate and lipid levels in cricket, possibly collaborative contribution with AKH to the maintenance of energy homeostasis.
脂肪动激素（AKH）通过动员脂肪体中的脂质和碳水化合物来调节昆虫的能量平衡。在此，我们利用RNA测序数据，在双斑蟋（Gryllus bimaculatus）的心侧体中鉴定出了编码AKH（GbAKH）及其高度同源的激素AKH/心钠素相关肽（GbACP）的cDNA。逆转录聚合酶链反应（RT-PCR）显示，GbAKH和GbACP分别主要在心侧体和咽侧体中表达。系统发育分析证实，鉴定出的GbAKH和GbACP分别属于包含其他AKH和ACP的进化枝。注射合成的GbAKH和GbACP可提高血淋巴中的碳水化合物和脂质水平，并显著减少食物摄入量。相反，通过RNA干扰敲低GbAKH和GbACP会增加食物摄入量，尽管血淋巴脂质水平没有改变。总的来说，这项研究提供了证据，表明ACP调节蟋蟀血淋巴中的碳水化合物和脂质水平，可能与AKH协同作用以维持能量平衡。
