Wen Ronghui, Ou Rong, Chen Baoshan
Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.
Virus Genes. 2007 Oct;35(2):443-8. doi: 10.1007/s11262-007-0086-z. Epub 2007 Mar 13.
Pieris rapae granulovirus (PiraGV) is a baculovirus pathogenic to the insect P. rapae (Pieridae, Lepidoptera). Though being known for decades, information on the genetic organization of this virus remains limited. In an effort to characterize this virus, an 11.8 kb BamHI restriction fragment that harbors the inhibitor of apoptosis gene (iap-5) was sequenced. Our results indicate that this region contains important genes such as dnapol, lef-3, lef-9, and dnaligase that are involved in transcription and replication of the virus. The gene content and synteny in this region are highly conserved among granulovirus genomes. Phylogenetic analysis showed that PiraGV genes are more closely related to the Choristoneura occidentalis granulovirus (ChocGV) than other characterized granulovirus (GVs).
菜青虫颗粒体病毒（PiraGV）是一种对菜青虫（粉蝶科，鳞翅目）致病的杆状病毒。尽管该病毒已为人所知数十年，但其基因组织信息仍然有限。为了对该病毒进行特征描述，对一个包含凋亡抑制基因（iap - 5）的11.8 kb BamHI限制性片段进行了测序。我们的结果表明，该区域包含参与病毒转录和复制的重要基因，如dnapol、lef - 3、lef - 9和dnaligase。该区域的基因含量和同线性在颗粒体病毒基因组中高度保守。系统发育分析表明，与其他已鉴定的颗粒体病毒（GVs）相比，PiraGV基因与西方云杉卷叶蛾颗粒体病毒（ChocGV）的关系更为密切。
