College of Life Sciences, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, China.
Arch Virol. 2011 Jul;156(7):1185-98. doi: 10.1007/s00705-011-0977-0. Epub 2011 Mar 26.
The complete genome of Clostera anachoreta granulovirus (ClanGV) from an important pest of poplar, Clostera anachoreta (Lepidoptera: Notodontidae), was sequenced and analyzed. The circular double-stranded genome is 101,487 bp in size with a C+G content of 44.4%. It is predicted to contain 123 open reading frames (ORFs), covering 93% of the whole genome sequence. One hundred eleven ClanGV ORFs are homologues of previously reported baculovirus genes, two ORFs only exist in nucleopolyhedroviruses (NPVs), and 10 ORFs are unique to ClanGV, accounting for 3.9% of the genome in total. The average amino acid sequence identities between ClanGV and other granulovirus (GV) homologues ranged from 55.5% (PiraGV) to 43.1% (HearGV and PsunGV). ClanGV includes 16 DNA replication- and transcription-related genes, including ie-1, dnapol, helicase, lef-1, lef-2, lef-3, lef-4, lef-5, lef-6, lef-8, lef-9, lef-10, lef-11, 39K, p47 and vlf-1. Seventeen conserved structural genes and two types of apoptosis suppression proteins (P35 and IAP) have also been identified. To date, ClanGV is the second identified GV that contains the p35 gene, which is present in eight NPVs and ChocGV. Genes of chitinase and cathepsin, which are involved in the liquefaction of the host, were found in the ClanGV genome, consistent with the typical insecticidal properties of ClanGV. In the ClanGV genome, there are four homologous repeat regions, each one of which contains only 2-3 direct repeats. Phylogenetic analysis, based on the 29 core baculovirus genes, indicates that ClanGV is closely related to PhopGV, AdorGV, CpGV, CrleGV, PiraGV and ChocGV, and this agrees with the results of homology analysis and gene parity plot analysis.
从杨扇舟蛾的重要害虫白杨潜叶蛾中分离出的杨扇舟蛾颗粒体病毒（ClanGV）的全基因组序列已被测序和分析。该环状双链基因组大小为 101487bp，GC 含量为 44.4%。预测它包含 123 个开放阅读框（ORFs），覆盖了整个基因组序列的 93%。111 个 ClanGV ORFs 是先前报道的杆状病毒基因的同源物，两个 ORFs 仅存在于核型多角体病毒（NPV）中，而 10 个 ORFs是 ClanGV 所特有的，占基因组的 3.9%。ClanGV 与其他颗粒体病毒（GV）同源物的平均氨基酸序列同一性范围为 55.5%（PiraGV）至 43.1%（HearGV 和 PsunGV）。ClanGV 包含 16 个与 DNA 复制和转录相关的基因，包括 ie-1、dnapol、解旋酶、lef-1、lef-2、lef-3、lef-4、lef-5、lef-6、lef-8、lef-9、lef-10、lef-11、39K、p47 和 vlf-1。还鉴定了 17 个保守结构基因和两种类型的凋亡抑制蛋白（P35 和 IAP）。迄今为止，ClanGV 是第二个鉴定出含有 p35 基因的 GV，该基因存在于八种 NPV 和 ChocGV 中。在 ClanGV 基因组中发现了几丁质酶和组织蛋白酶基因，它们参与宿主的液化，这与 ClanGV 的典型杀虫特性一致。在 ClanGV 基因组中，有四个同源重复区域，每个区域只包含 2-3 个直接重复。基于 29 个核心杆状病毒基因的系统发育分析表明，ClanGV 与 PhopGV、AdorGV、CpGV、CrleGV、PiraGV 和 ChocGV 密切相关，这与同源分析和基因均等性图谱分析的结果一致。
