Meneses Allan, Flores Dora, Muñoz Miguel, Arrieta Griselda, Espinoza Ana M
Centro de Investigación en Biología Celular y Molecular. Universidad de Costa Rica, San José. Costa Rica.
Rev Biol Trop. 2005 Sep-Dec;53(3-4):361-8.
With the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety CR-5272 (Oryza sativa L.), two independent experiments were performed. The first experiment consisted in the effect of combination of three concentrations of the gelling agent Phytagel (1.8. 2.4. and 3 gL(-1)) and four 2,4-D concentrations (2.26, 4.52. 6.78, and 9.05 microM) on the induction and subsequent regeneration of embryogenic calli. On the second experiment, the pre-regeneration phase was modified: calli were subjected to darkness or diffuse light conditions for one, two, and three weeks. In embryogenesis induction, 35% calligenesis was obtained using the MS culture medium supplemented with 6.78 microM of 2.4-D and 2.4 gL(-1) Phytagel, whereas on the control treatment (MS medium supplemented with 9.05 microM of 2.4-D and 3 gL(-1) Phytagel ) 24% calligenesis was obtained. In addition, regeneration percentages were improved (22% and 16% for calli induced with the above treatments, respectively). Furthermore, in light exposure experiments, the best result was obtained by exposing the embryogenic calli to darkness for one week in pre-regeneration, followed by direct light exposure during the regeneration phase.
为了提高从籼稻品种CR - 5272（Oryza sativa L.）的体细胞胚获得的离体植株的胚胎发生再生过程，进行了两项独立实验。第一个实验研究了三种浓度的胶凝剂Phytagel（1.8、2.4和3 gL(-1)）与四种2,4 - D浓度（2.26、4.52、6.78和9.05 microM）的组合对胚性愈伤组织诱导及后续再生的影响。在第二个实验中，对预再生阶段进行了改良：将愈伤组织置于黑暗或漫射光条件下1周、2周和3周。在胚胎发生诱导方面，使用添加了6.78 microM 2,4 - D和2.4 gL(-1) Phytagel的MS培养基获得了35%的愈伤组织诱导率，而在对照处理（添加9.05 microM 2,4 - D和3 gL(-1) Phytagel的MS培养基）中，愈伤组织诱导率为24%。此外，再生率有所提高（上述处理诱导的愈伤组织再生率分别为22%和16%）。此外，在光照实验中，最佳结果是在预再生阶段将胚性愈伤组织置于黑暗中1周，然后在再生阶段直接光照。
