Ochi M, Ikuta Y, Miyamoto Y, Takeno S
Department of Orthopaedic Surgery, Hiroshima University School of Medicine, Japan.
Exp Neurol. 1992 Feb;115(2):260-5. doi: 10.1016/0014-4886(92)90060-4.
Studies on axonal regeneration in Y-chambers over the past decade have consistently provided evidence of preferential growth toward a distal nerve piece. However, these findings are in contrast to the observations by Weiss and Taylor (1944. J. Exp. Zool. 95: 233-257), indicating that in fresh aortic Y-chambers regenerating axons had no tendency to grow into a channel containing a distal nerve piece as compared to an open channel. The discrepancy between Weiss and Taylor's findings and those of later authors remains unexplained. In the present study we repeated the investigations by Weiss and Taylor, using both isogenic and allogenic aortic Y-chambers. In control groups the aorta graft was frozen and treated chemically to kill all cells and to deactivate possible growth factors of protein nature. In these groups preferential growth toward the distal nerve was pronounced although such specificity in growth was evident also in the nontreated types of aortic chambers. The findings do not support the results presented by Weiss and Taylor.
在过去十年间，对Y型腔室中轴突再生的研究一直都能提供证据，证明轴突会优先朝着远端神经片段生长。然而，这些研究结果与魏斯和泰勒（1944年，《实验动物学杂志》95卷：233 - 257页）的观察结果相反，他们指出，在新鲜的主动脉Y型腔室中，与开放通道相比，再生轴突没有生长到包含远端神经片段的通道中的倾向。魏斯和泰勒的研究结果与后来研究者的结果之间的差异仍未得到解释。在本研究中，我们重复了魏斯和泰勒的研究，使用了同基因和异基因主动脉Y型腔室。在对照组中，将主动脉移植物冷冻并进行化学处理，以杀死所有细胞并使可能的蛋白质性质的生长因子失活。在这些组中，尽管在未经处理的主动脉腔室类型中生长的这种特异性也很明显，但向远端神经的优先生长很显著。这些研究结果不支持魏斯和泰勒所呈现的结果。
