Dauguet Julien, Peled Sharon, Berezovskii Vladimir, Delzescaux Thierry, Warfield Simon K, Born Richard, Westin Carl-Fredrik
Computational Radiology Laboratory, Children's Hospital, Brigham and Women's Hospital, Harvard Medical School, Boston, USA.
Med Image Comput Comput Assist Interv. 2006;9(Pt 1):109-16. doi: 10.1007/11866565_14.
A classical neural tract tracer, WGA-HRP, was injected at multiple sites within the brain of a macaque monkey. Histological sections of the labeled fiber tracts were reconstructed in 3D, and the fibers were segmented and registered with the anatomical post-mortem MRI from the same animal. Fiber tracing along the same pathways was performed on the DTI data using a classical diffusion tracing technique. The fibers derived from the DTI were compared with those segmented from the histology in order to evaluate the performance of DTI fiber tracing. While there was generally good agreement between the two methods, our results reveal certain limitations of DTI tractography, particularly at regions of fiber tract crossing or bifurcation.
一种经典的神经束示踪剂，即小麦胚芽凝集素-辣根过氧化物酶（WGA-HRP），被注射到一只猕猴大脑内的多个部位。对标记纤维束的组织学切片进行三维重建，然后对纤维进行分割，并与同一动物的解剖后磁共振成像（MRI）进行配准。使用经典的扩散追踪技术，在扩散张量成像（DTI）数据上沿相同路径进行纤维追踪。将DTI得出的纤维与从组织学中分割出的纤维进行比较，以评估DTI纤维追踪的性能。虽然这两种方法总体上有较好的一致性，但我们的结果揭示了DTI纤维束成像的某些局限性，特别是在纤维束交叉或分叉的区域。
