利用高通量 MRI 对小鼠边缘下区和额前皮质连接进行 DTI 束路追踪分析。
A DTI tractography analysis of infralimbic and prelimbic connectivity in the mouse using high-throughput MRI.
机构信息
Department of Biomedical Informatics, Emory University, USA.
出版信息
Neuroimage. 2012 Nov 1;63(2):800-11. doi: 10.1016/j.neuroimage.2012.07.014. Epub 2012 Jul 14.
BACKGROUND
High throughput, brain-wide analysis of neural circuit connectivity is needed to understand brain function across species. Combining such tractography techniques with small animal models will allow more rapid integration of systems neuroscience with molecular genetic, behavioral, and cellular approaches.
METHODS
We collected DTI and T2 scans on 3 series of 6 fixed mouse brains ex vivo in a 9.4 Tesla magnet. The DTI analysis of ten mouse brains focused on comparing prelimbic (PL) and Infralimbic (IL) probabilistic tractography. To validate the DTI results a preliminary set of 24 additional mice were injected with BDA into the IL and PL. The DTI results and preliminary BDA results were also compared to previously published rat connectivity.
RESULTS
We focused our analyses on the connectivity of the mouse prelimbic (PL) vs. infralimbic (IL) cortices. We demonstrated that this DTI analysis is consistent across scanned mice, with prior analyses of rat IL/PL connectivity, and with mouse PL and IL projections using the BDA tracer.
CONCLUSIONS
High-throughput ex vivo DTI imaging in the mouse delineated both common and differential connectivity of the IL and PL cortex. The scanning methodology provided a balance of tissue contrast, signal-to-noise ratio, resolution and throughput. Our results are largely consistent with previously published anterograde staining techniques in rats, and the preliminary tracer study of the mouse IL and PL provided here.
背景
为了了解跨物种的大脑功能，需要高通量、全脑分析神经回路连接。将这种示踪技术与小动物模型相结合，将使系统神经科学更迅速地与分子遗传学、行为学和细胞学方法结合。
方法
我们在 9.4T 磁体中对 3 组 6 只固定的离体鼠脑进行了 DTI 和 T2 扫描。对 10 只鼠脑的 DTI 分析侧重于比较额前皮质（PL）和下边缘皮质（IL）的概率示踪。为了验证 DTI 结果，对初步的 24 只老鼠进行了 IL 和 PL 注射 BDA 的实验。DTI 结果和初步的 BDA 结果也与之前发表的大鼠连接进行了比较。
结果
我们的分析集中在鼠的前扣带皮质（PL）与下边缘皮质（IL）的连接上。我们证明，这种 DTI 分析在扫描的老鼠之间是一致的，与之前的大鼠 IL/PL 连接的分析一致，与使用 BDA 示踪剂的鼠 PL 和 IL 投射一致。
结论
在离体鼠中进行高通量的 DTI 成像，描绘了 IL 和 PL 皮质的共同和差异连接。扫描方法提供了组织对比度、信噪比、分辨率和吞吐量的平衡。我们的结果与之前发表的大鼠顺行染色技术和这里初步的鼠 IL 和 PL 示踪研究大致一致。
Zotero 插件