Noguchi Masafumi, Aoki Eisuke, Yoshida Daiki, Kobayashi Etsuko, Omori Shigeru, Muragaki Yoshihiro, Iseki Hiroshi, Nakamura Katsushige, Sakuma Ichiro
Graduate School of Frontier Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Med Image Comput Comput Assist Interv. 2006;9(Pt 1):543-50. doi: 10.1007/11866565_67.
We developed a combined system of tumor detection by 5-ALA-induced PpIX fluorescence and precise ablation by micro laser for the first time, with an automatic focusing and robotic scanning mechanism for the brain surface. 5-ALA accumulates on tumors to be metabolized to become PpIX that is a fluorescent. Intra-operative detection of 5-ALA induced PpIX fluorescence provides useful information for tumor detection. The wavelength of the micro laser is 2.8 microm close to the absorption band of water. This laser is effective only on the surface of brain tissue, enabling precise ablation at the boundary between tumor and normal tissue identified by intra-operative 5-ALA induced fluorescence. Combination tests of the fluorescence measurement and the laser ablation were performed, and it was possible to extract the area with fluorescence appropriately from the measurement data, and the micro laser with automatically scanning selectively ablated the extracted area.
我们首次开发了一种结合系统，通过5-氨基乙酰丙酸（5-ALA）诱导的原卟啉IX（PpIX）荧光进行肿瘤检测，并通过微激光进行精确消融，该系统具有用于脑表面的自动聚焦和机器人扫描机制。5-ALA在肿瘤上积累并代谢成为具有荧光的PpIX。术中检测5-ALA诱导的PpIX荧光可为肿瘤检测提供有用信息。微激光的波长为2.8微米，接近水的吸收带。这种激光仅对脑组织表面有效，能够在术中5-ALA诱导荧光识别的肿瘤与正常组织边界处进行精确消融。进行了荧光测量和激光消融的联合测试，能够从测量数据中适当地提取出有荧光的区域，并且自动扫描的微激光选择性地消融了提取出的区域。
