从法国分离的泰氏泰勒菌 23S 核糖体 RNA（rRNA）基因内内含子（IVS）的分子鉴定和特征分析。
Molecular identification and characterization of the intervening sequences (IVSs) within 23S ribosomal RNA (rRNA) genes of Taylorella asinigenitalis isolated in France.
机构信息
School of Environmental Health Sciences, Azabu University, Chuo-ku Sagamihara, Japan.
出版信息
Res Vet Sci. 2012 Feb;92(1):45-52. doi: 10.1016/j.rvsc.2010.10.004. Epub 2010 Nov 13.
In the helix 25 region, 32 French Taylorella asinigenitalis isolates carried at least one 23S rRNA gene not containing intervening sequences (IVSs). No IVSs in the region were identified in three isolates and the other remaining 29 isolates carried one or more IVSs (UCD-1(T)IVS1A, UCD-1(T)IVS1B and UK-1IVS1B) described already and two new kinds of IVS (TaIVS1C and TaIVS1D). In the helix 45 region, no T. asinigenitalis isolates not carrying any IVSs were identified. UK-1IVS2B was identified in the region from 26 isolates. Five new kinds of IVSs (TaIVS2D, E, F, G and H) occurred in the region in the 13 isolates. Distinctly different tandem repeat units (RS48 and RS32 and RS-A, -B and -C) were evident in both regions, respectively, from the French (n=32) and American (n=3) T. asinigenitalis isolates. Thus, several different kinds of tandem repeat units and their combinations in IVSs in both regions within the gene were shown in 32 French isolates.
在 helix 25 区域，32 株法国泰勒雷拉阴道亚种分离株至少携带一个不含有间插序列（IVS）的 23S rRNA 基因。在三个分离株中未发现该区域的 IVS，而其余 29 个分离株携带一个或多个已描述的 IVS（UCD-1(T)IVS1A、UCD-1(T)IVS1B 和 UK-1IVS1B）以及两种新的 IVS（TaIVS1C 和 TaIVS1D）。在 helix 45 区域，未发现未携带任何 IVS 的 T. asinigenitalis 分离株。从 26 个分离株中鉴定出了 UK-1IVS2B。在该区域的 13 个分离株中发现了五种新的 IVS（TaIVS2D、E、F、G 和 H）。在法国（n=32）和美国（n=3）的 T. asinigenitalis 分离株中，分别在这两个区域中发现了明显不同的串联重复单元（RS48 和 RS32 和 RS-A、-B 和 -C）。因此，在 32 株法国分离株中，这两个区域的基因内都显示了几种不同类型的串联重复单元及其 IVS 组合。
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