Simmons Cameron P, Popper Stephen, Dolocek Christiane, Chau Tran Nguyen Bich, Griffiths Michael, Dung Nguyen Thi Phuong, Long Truong Hoang, Hoang Dang Minh, Chau Nguyen Vinh, Thao Le Thi Thu, Hien Tran Tinh, Relman David A, Farrar Jeremy
Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
J Infect Dis. 2007 Apr 15;195(8):1097-107. doi: 10.1086/512162. Epub 2007 Mar 5.
Responses by peripheral blood leukocytes may contribute to the pathogenesis of dengue hemorrhagic fever (DHF). We used DNA microarrays to reveal transcriptional patterns in the blood of 14 adults with DHF. Acute DHF was defined by an abundance of transcripts from cell cycle- and endoplasmic reticulum (ER)-related genes, suggesting a proliferative response accompanied by ER stress. Transcript-abundance levels for immunoresponse-associated genes, including cell surface markers, immunoglobulin, and innate response elements, were also elevated. Twenty-four genes were identified for which transcript abundance distinguished patients with dengue shock syndrome (DSS) from those without DSS. All the gene transcripts associated with DSS, many of which are induced by type I interferons, were less abundant in patients with DSS than in those without DSS. To our knowledge, these data provide the first snapshot of gene-expression patterns in peripheral blood during acute dengue and suggest that DSS is associated with attenuation of selected aspects of the innate host response.
外周血白细胞的反应可能在登革出血热（DHF）的发病机制中起作用。我们使用DNA微阵列来揭示14名成年DHF患者血液中的转录模式。急性DHF的定义是细胞周期和内质网（ER）相关基因的转录本丰富，提示伴有ER应激的增殖反应。免疫反应相关基因的转录本丰度水平也升高，包括细胞表面标志物、免疫球蛋白和固有反应元件。鉴定出24个基因，其转录本丰度可区分登革休克综合征（DSS）患者和非DSS患者。所有与DSS相关的基因转录本，其中许多由I型干扰素诱导，在DSS患者中的丰度低于非DSS患者。据我们所知，这些数据提供了急性登革热期间外周血基因表达模式的首张快照，并表明DSS与固有宿主反应某些方面的选择相关。
