Mounts P, Melton C G
Experientia. 1975 Nov 15;31(11):1343-5. doi: 10.1007/BF01945817.
Rotenone and high doses of chloramphenicol, both of which specifically inhibit electron transport between NADH and flavoprotein in the respiratory chain, caused fully separated Rana pipiens blastomeres to refuse, as shown by syncytium counts on embryos reconstructed from serial sections. With chloramphenicol, the effect was completely reversible: re-cleavage and normal development followed drug removal. The blastomere fusion effect was not produced by the succinic dehydrogenase-specific respiratory inhibitor, thenoyltrifluoroacetone, nor by a non-mitochondrial protein synthesis inhibitor, cycloheximide, both of which instead produced simple arrest of cleavage.
鱼藤酮和高剂量的氯霉素都能特异性抑制呼吸链中NADH和黄素蛋白之间的电子传递，如通过对连续切片重建的胚胎进行合胞体计数所示，这两种物质会导致完全分离的豹蛙卵裂球停止分裂。使用氯霉素时，这种作用是完全可逆的：去除药物后，胚胎会重新分裂并正常发育。琥珀酸脱氢酶特异性呼吸抑制剂噻吩甲酰三氟丙酮以及非线粒体蛋白质合成抑制剂环己酰亚胺均未产生卵裂球融合效应，相反，这两种物质都只会导致简单的卵裂停滞。
