Castell L M
University Department of Pharmacology, Oxford, U.K.
J Immunol Methods. 1992 Jan 21;146(1):49-53. doi: 10.1016/0022-1759(92)90047-w.
A simple and inexpensive method to obtain substantially platelet-free and erythrocyte-free lymphocyte preparations from human peripheral blood has been established, using a modification of the technique published by Vives et al. (1971). Lymphocyte levels of up to 3 x 10(6) per ml blood have been obtained, with 2-3% platelet and erythrocyte contamination per ml blood, by pre-aggregating platelets with low concentrations of adenosine 5'diphosphate (ADP).
利用对维韦斯等人（1971年）发表的技术进行改进的方法，已建立了一种从人外周血中获得基本上无血小板和无红细胞的淋巴细胞制剂的简单且廉价的方法。通过用低浓度的5'-二磷酸腺苷（ADP）预聚集血小板，每毫升血液中已获得高达3×10⁶的淋巴细胞水平，每毫升血液中血小板和红细胞的污染率为2%-3%。
