一种从少量小鼠外周血中分离淋巴细胞的改良技术。
An improved technique for the isolation of lymphocytes from small volumes of peripheral mouse blood.
作者信息
Mizobe F, Martial E, Colby-Germinario S, Livett B G
出版信息
J Immunol Methods. 1982;48(3):269-79. doi: 10.1016/0022-1759(82)90327-1.
PMID:7061865
Abstract
Centrifugation of mouse blood on density gradients of polyvinylpyrrolidone-coated colloidal silica (Percoll) resulted in the simultaneous separation of platelets, lymphocytes, granulocytes and erythrocytes. By this method, we have been successful in isolating a fraction of highly purified and viable lymphocytes from small volumes of peripheral mouse blood with good recovery.
摘要
在聚乙烯吡咯烷酮包被的胶体二氧化硅（Percoll）密度梯度上对小鼠血液进行离心，可同时分离血小板、淋巴细胞、粒细胞和红细胞。通过这种方法，我们成功地从小鼠外周血小体积样本中分离出了一部分高度纯化且具有活力的淋巴细胞，回收率良好。
Zotero 插件