Longoni P, Rota Scalabrini D, Cascone G, Veroni C, Lo Faro A
Boll Soc Ital Biol Sper. 1984 Mar 30;60(3):649-55.
We studied the ultrastructural morphology and function of platelets collected by apheresis procedure with discontinuous flow using the Surge Pump Technique. The platelet concentrates obtained by this technique are free of erythrocyte and lymphocyte contamination. The platelet morphology as well as the platelet aggregation induced by ADP, adrenaline and collagen of platelet concentrates were similar to those observed in PRP before the apheresis. The response to the hypotonic shock of platelet concentrates was also normal, indicating membrane integrity and good platelet metabolism. These results show that platelet concentrates obtained by the Surge Pump Technique maintain their haemostatic effects and may be infused in thrombocitopenic patients, reducing the risk of alloimmunization related to the presence of erythrocytes and lymphocytes.
我们使用浪涌泵技术研究了通过间断流动单采程序采集的血小板的超微结构形态和功能。通过该技术获得的血小板浓缩物不含红细胞和淋巴细胞污染。血小板浓缩物的血小板形态以及由ADP、肾上腺素和胶原蛋白诱导的血小板聚集与单采前在富血小板血浆中观察到的相似。血小板浓缩物对低渗休克的反应也正常，表明膜完整性良好且血小板代谢正常。这些结果表明，通过浪涌泵技术获得的血小板浓缩物保持了其止血效果，可输注给血小板减少症患者，降低与红细胞和淋巴细胞存在相关的同种免疫风险。
