Li Yaolan, Jiang Renwang, Ooi Linda S M, But Paul P H, Ooi Vincent E C
Department of Chemistry, Jinan University, Guangzhou, Guangdong, China.
Phytother Res. 2007 May;21(5):466-70. doi: 10.1002/ptr.1962.
Schefflera heptaphylla (L.) Frodin is a principal ingredient of an herbal tea formulation widely used for the treatment of common cold in southern China. An extract of the long leafstalk of the compound leaf of S. heptaphylla exhibited the most potent antiviral activity against respiratory syncytial virus (RSV). Further antiviral-guided fractionation and isolation of the leafstalk extract of S. heptaphylla led to obtain two highly active pure triterpenoids, namely 3alpha-hydroxylup-20(29)-ene-23,28-dioic acid and 3-epi-betulinic acid 3-O-sulfate, together with an inactive saponin, 3alpha-hydroxylup-20(29)-ene-23,28-dioic acid 28-O-alpha-l-rhamnopyranosyl-(1-->4)-O-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranoside. An antiviral assay using a cytopathic effect (CPE) reduction method showed that the two triterpenoids possessed broader antiviral activity against respiratory syncytial virus (RSV) with a similar 50% inhibition concentration (IC(50)) value of 6.25 microg/mL, influenza A (H1N1) virus with IC(50) values of 25 and 31.3 microg/mL, Coxsackie B3 (Cox B3) virus with IC(50) values of 12.5 and 20 microg/mL and herpes simplex virus type 1 (HSV-1) with IC(50) values of 18.8 and 25 microg/mL, respectively, whereas the saponin did not have antiviral activity against these four viruses at a concentration of 100 microg/mL.
鹅掌柴（Schefflera heptaphylla (L.) Frodin）是一种草药茶配方的主要成分，在中国南方广泛用于治疗普通感冒。鹅掌柴复叶长叶柄的提取物对呼吸道合胞病毒（RSV）表现出最强的抗病毒活性。对鹅掌柴叶柄提取物进行进一步的抗病毒导向分级分离和分离，得到了两种高活性的纯三萜类化合物，即3α-羟基羽扇-20(29)-烯-23,28-二酸和3-表-桦木酸3-O-硫酸盐，以及一种无活性的皂苷，3α-羟基羽扇-20(29)-烯-23,28-二酸28-O-α-L-鼠李糖基-(1→4)-O-β-D-葡萄糖基-(1→6)-β-D-葡萄糖苷。采用细胞病变效应（CPE）降低法进行的抗病毒试验表明，这两种三萜类化合物对呼吸道合胞病毒（RSV）具有更广泛的抗病毒活性，其50%抑制浓度（IC50）值相似，为6.25μg/mL，对甲型流感（H1N1）病毒的IC50值分别为25和31.3μg/mL，对柯萨奇B3（Cox B3）病毒的IC50值分别为12.5和20μg/mL，对单纯疱疹病毒1型（HSV-1）的IC50值分别为18.8和25μg/mL，而该皂苷在100μg/mL浓度下对这四种病毒均无抗病毒活性。
