Park Jongman, Lee Dami, Kim Won, Horiike Shigeyoshi, Nishimoto Takahiro, Lee Se Hwan, Ahn Chong H
Analytical Laboratory, Department of Chemistry, Konkuk University, 1 Hwayangdong, Gwangjingu, Seoul 143-701, Korea.
Anal Chem. 2007 Apr 15;79(8):3214-9. doi: 10.1021/ac061714g. Epub 2007 Mar 15.
A fully packed capillary electrochromatographic (CEC) microchip showing improved solution and chip handling was developed. Microchannels for the CEC microchip were patterned on a cyclic olefin copolymer substrate by injection molding and packed fully with 0.8-microm monodisperse colloidal silica beads utilizing a self-assembly packing technique. The silica packed chip substrate was covered and thermally press-bonded. After fabrication, the chip was filled with buffer solution by self-priming capillary action. The self-assembly packing at each channel served as a built-in nanofilter allowing quick loading of samples and running buffer solution without filtration. Because of a large surface area-to-volume ratio of the silica packing, reproducible control of electroosmotic flow was possible without leveling of the solutions in the reservoirs resulting 1.3% rsd in migration rate. The capillary electrophoretic separation characteristics of the chip were studied using fluorescein isothiocyanate (FITC)-derivatized amino acids as probe molecules. A mixture of FITC and four FITC-derivatized amino acids was successfully separated with 2-mm separation channel length.
开发出了一种填充完全的毛细管电色谱（CEC）微芯片，其在溶液和芯片处理方面表现出改进。CEC微芯片的微通道通过注塑成型在环烯烃共聚物基板上进行图案化，并利用自组装填充技术用0.8微米的单分散胶体二氧化硅珠完全填充。填充有二氧化硅的芯片基板被覆盖并热压键合。制造完成后，芯片通过自吸毛细管作用填充缓冲溶液。每个通道的自组装填充充当内置纳米过滤器，允许在不进行过滤的情况下快速加载样品和运行缓冲溶液。由于二氧化硅填充物的表面积与体积之比很大，无需在储液器中使溶液水平一致就能实现电渗流的可重复控制，迁移率的相对标准偏差为1.3%。使用异硫氰酸荧光素（FITC）衍生的氨基酸作为探针分子研究了芯片的毛细管电泳分离特性。在2毫米的分离通道长度下，成功分离了FITC和四种FITC衍生的氨基酸的混合物。
