Interdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611-6250, USA.
Analyst. 2011 Apr 7;136(7):1288-97. doi: 10.1039/c0an00969e. Epub 2011 Jan 28.
Microfluidics is a platform technology that has been used for genomics, proteomics, chemical synthesis, environment monitoring, cellular studies, and other applications. The fabrication materials of microfluidic devices have traditionally included silicon and glass, but plastics have gained increasing attention in the past few years. We focus this review on thermoplastic microfluidic devices and their applications in protein and DNA analysis. We outline the device design and fabrication methods, followed by discussion on the strategies of surface treatment. We then concentrate on several significant advancements in applying thermoplastic microfluidic devices to protein separation, immunoassays, and DNA analysis. Comparison among numerous efforts, as well as the discussion on the challenges and innovation associated with detection, is presented.
微流控是一种平台技术，已被用于基因组学、蛋白质组学、化学合成、环境监测、细胞研究等领域。微流控器件的传统制造材料包括硅和玻璃，但近年来塑料受到了越来越多的关注。我们重点关注热塑性微流控器件及其在蛋白质和 DNA 分析中的应用。我们概述了器件的设计和制造方法，然后讨论了表面处理策略。接着，我们集中讨论了将热塑性微流控器件应用于蛋白质分离、免疫分析和 DNA 分析的一些重要进展。我们比较了众多研究成果，并讨论了与检测相关的挑战和创新。
