Laachi Nabil, Declet Carmelo, Matson Christina, Dorfman Kevin D
Department of Chemical Engineering and Materials Science, University of Minnesota, Twin Cities, 421 Washington Avenue SE, Minneapolis, Minnesota 55455, USA.
Phys Rev Lett. 2007 Mar 2;98(9):098106. doi: 10.1103/PhysRevLett.98.098106. Epub 2007 Mar 1.
We consider theoretically the dynamics of short duplex DNA during high-field electrophoresis through a periodic array of narrow slits and deep wells (a nanofilter), where the slit depth is less than the contour length of the essentially rigid DNA strand. In contrast with the known behavior under weak fields, we predict that the larger chains will elute first under strong electric fields via "torque-assisted escape" from the wells. This contradicts the maxim that separations must be performed close to equilibrium, and opens the way for enhanced nanofluidic separations of DNA based upon their out-of-equilibrium transport properties.
我们从理论上考虑了短双链DNA在通过周期性排列的窄缝和深阱（纳米过滤器）的高场电泳过程中的动力学，其中缝的深度小于基本刚性的DNA链的轮廓长度。与弱场下的已知行为相反，我们预测在强电场下，较大的链将通过从阱中的“扭矩辅助逃逸”率先洗脱。这与分离必须在接近平衡的条件下进行这一准则相矛盾，并为基于DNA非平衡传输特性的增强型纳米流体分离开辟了道路。
