利用聚合酶链反应检测圈养和野生黑猩猩（Pan troglodytes）粪便中的产气荚膜梭菌。
Polymerase chain reaction detection of Clostridium perfringens in feces from captive and wild chimpanzees, Pan troglodytes.
作者信息
Fujita Shiho, Kageyama Takashi
机构信息
Laboratory of Research Resource, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Tsukuba, Japan.
出版信息
J Med Primatol. 2007 Feb;36(1):25-32. doi: 10.1111/j.1600-0684.2006.00191.x.
BACKGROUND
For veterinary management of non-human primates in captivity, and conservation of wild-living primates, management of their health risks is necessary. Incidences of pathogenic bacteria in the fecal specimens are considered as one of the useful indicators for non-invasive health monitoring.
METHODS
We carried out the detection of Clostridium perfringens in feces from captive and wild chimpanzees by the rapid polymerase chain reaction method.
RESULTS
The bacterium was detected in most fecal specimens (80%) in captive chimpanzees. Contrarily, the detection rate in the wild chimpanzees was low, with 23% (n = 12) of 53 fecal samples from the Bossou group, Guinea, and 1.2% (1/81) in the Mahale group, Tanzania.
CONCLUSIONS
These results show that the intestinal microflora differs between Pan populations under various living conditions, being influenced by their diet and environment.
背景
对于圈养非人灵长类动物的兽医管理以及野生灵长类动物的保护而言，管理其健康风险是必要的。粪便样本中病原菌的发生率被视为非侵入性健康监测的有用指标之一。
方法
我们采用快速聚合酶链反应法对圈养和野生黑猩猩粪便中的产气荚膜梭菌进行检测。
结果
圈养黑猩猩的大多数粪便样本（80%）中检测到了该细菌。相反，野生黑猩猩的检出率较低，几内亚博苏群体的53份粪便样本中有23%（n = 12）检出，坦桑尼亚马哈勒群体的检出率为1.2%（1/81）。
结论
这些结果表明，不同生活条件下的泛种群肠道微生物群存在差异，受到饮食和环境的影响。
Zotero 插件