Chae Younbyoung, Hong Mee-Suk, Kim Gun-Ho, Hahm Dae-Hyun, Park Hae-Jeong, Ha Eunyoung, Kim Mi-Ja, Park Hi-Joon, Yang Jongsoo, Lee Hyejung
Acupuncture and Meridian Science Research Center, Kyung Hee University, Seoul 130-701, Korea.
Neurol Res. 2007;29 Suppl 1:S55-8. doi: 10.1179/016164107X172365.
The aim of this study was to investigate the anti-inflammatory effect of acupuncture on acute paw edema induced by carrageenan (CR) injection and to detect differential cytokine responses in response to acupuncture stimulation using protein array technology.
Control group was injected with CR (1%, 50 mul) into the plantar surface of the male Sprague-Dawley rats. Acupuncture group was stimulated with acupuncture at Zusanli (ST36) 30 minutes after CR injection. Rat cytokine antibody array coated with 19 specific cytokine antibodies were probed with protein samples and the relative cytokine levels were investigated.
Acupuncture stimulation significantly inhibited the inflammatory response to CR injection. Compared to control group, three cytokines, interleukin-6 (IL-6), beta-nerve growth factor (beta-NGF) and tissue inhibitors of metalloproteinase-1 (TIMP-1), showed significantly decreased expression levels in the acupuncture group. The other 16 cytokines did not exhibit significant changes between two groups.
These results indicate that acupuncture markedly inhibited CR-induced edema and modulated the expressions of certain cytokines in response to CR-induced inflammation. These findings might give us a clue in elucidating the underlying mechanism of anti-inflammatory effect of acupuncture.
本研究旨在探讨针刺对角叉菜胶（CR）注射所致急性足爪水肿的抗炎作用，并使用蛋白质芯片技术检测针刺刺激后细胞因子的差异反应。
将CR（1%，50微升）注射到雄性Sprague-Dawley大鼠的足底，作为对照组。CR注射30分钟后，针刺足三里（ST36）对针刺组进行刺激。用蛋白质样品检测包被有19种特异性细胞因子抗体的大鼠细胞因子抗体芯片，并研究相关细胞因子水平。
针刺刺激显著抑制了对CR注射的炎症反应。与对照组相比，针刺组中三种细胞因子，即白细胞介素-6（IL-6）、β-神经生长因子（β-NGF）和金属蛋白酶组织抑制剂-1（TIMP-1）的表达水平显著降低。其他16种细胞因子在两组之间未表现出显著变化。
这些结果表明，针刺显著抑制了CR诱导的水肿，并调节了对CR诱导炎症反应的某些细胞因子的表达。这些发现可能为阐明针刺抗炎作用的潜在机制提供线索。
