Shin Sehyun, Ku Yun-Hee, Ho Jian-Xun, Kim Yu-Kyung, Suh Jang-Soo, Singh Megha
School of Mechanical Engineering, Kyungpook National University, Daegu 702-701, Korea.
Clin Hemorheol Microcirc. 2007;36(3):253-61.
The erythrocyte deformability of blood samples, of diabetes mellitus (DM) patients with and without microangiopathic complications such as nephropathy and retinopathy, is determined and is compared with that of healthy control. The erythrocyte deformability is measured in terms of elongation index (EI) with microfluidic ektacytometer, which is very sensitive to detect changes in EI of erythrocytes due to hyperglycemic process. Each measurement of diffraction pattern of erythrocyte suspension in a highly viscous polyvinyl pyrroridone (PVP) solution in a disposable microchannel is carried out. The results show that EI is well correlated with the levels of glycated hemoglobin and creatinine, as determined from the blood samples of patients. A significant decrease in the EI in DM patients compared with that in normal control is observed. In patients with complications of chronic renal failure, end stage renal disease, retinopathy and with combination of retinopathy and nephropathy, the EI is significantly decreased in comparison with that of diabetes patients without these complications. Further reduction in EI is corresponded to the changes induced by the microangiopathy process despite the maintenance of blood glucose and glycated hemoglobin by drug therapy.
对患有和未患有诸如肾病和视网膜病变等微血管并发症的糖尿病（DM）患者的血样进行红细胞变形性测定，并与健康对照者的进行比较。使用微流控血细胞变形仪通过伸长指数（EI）来测量红细胞变形性，该仪器对检测由于高血糖过程导致的红细胞EI变化非常敏感。在一次性微通道中对红细胞悬液在高粘性聚乙烯吡咯烷酮（PVP）溶液中的衍射图案进行每次测量。结果表明，EI与从患者血样中测定的糖化血红蛋白和肌酐水平密切相关。观察到DM患者的EI与正常对照相比显著降低。在患有慢性肾衰竭、终末期肾病、视网膜病变以及视网膜病变和肾病合并症的患者中，与没有这些并发症的糖尿病患者相比，EI显著降低。尽管通过药物治疗维持了血糖和糖化血红蛋白水平，但EI的进一步降低与微血管病变过程引起的变化相对应。
