Grzelak A, Skierski J, Bartosz G
Department of Molecular Biophysics, University of Lłodź, Poland.
FEBS Lett. 2001 Mar 9;492(1-2):123-6. doi: 10.1016/s0014-5793(01)02244-x.
Replicatively senescent cells of Saccharomyces cerevisiae were obtained using the 'baby machine' method by immobilizing cells on CovaLink NH(2) plates and allowing them to divide while exchanging medium and removing daughter cells. Centrifugation in a Percoll density gradient was employed for further purification of replicatively old yeast cells. Comparison of senescent cells showing more than 20 bud scars with cells from early stationary culture demonstrated a significant reduction of total and reduced glutathione and decrease of superoxide dismutase activity during replicative aging of yeast cells.
使用“幼芽机器”方法，通过将酿酒酵母细胞固定在CovaLink NH(2)平板上，并在更换培养基和去除子细胞的同时让它们分裂，获得了复制性衰老细胞。采用在Percoll密度梯度中离心的方法进一步纯化复制性衰老的酵母细胞。将显示超过20个芽痕的衰老细胞与早期稳定期培养的细胞进行比较，结果表明，在酵母细胞的复制性衰老过程中，总谷胱甘肽和还原型谷胱甘肽显著减少，超氧化物歧化酶活性降低。
