用C带和原位杂交技术对白跗按蚊（双翅目：蚊科）进行细胞遗传学研究。

Cytogenetic study of Anopheles albitarsis (Diptera: Culicidae) by C-banding and in situ hybridization.

作者信息

Rafael M S, Santos I P, Tadei W P, Carvalho K A, Recco-Pimente S M, Sallum M A M, Forattini O P

机构信息

Coordenação de Pesquisas em Ciências da Saúde, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brazil.

出版信息

Hereditas. 2006 Dec;143(2006):62-7. doi: 10.1111/j.2006.0018-0661.01926.x.

DOI:10.1111/j.2006.0018-0661.01926.x

PMID:17362336

Abstract

The C-banding pattern and the size and location of the nucleolar organizer regions (NORs) are described for the first time in Brazilian populations of Anopheles (Nyssorhynchus) albitarsis sensu lato. C-banding revealed variation in the size of the centromeric heterochromatic blocks in autosomal chromosomes and in the acrocentric (X) and puntiform (Y) sex chromosomes. Fluorescence in situ hybridization showed that the NORs were located in the pericentromeric region of the sex (XX/XY) chromosomes and that this coincided with the number and location of centromeric constitutive heterochromatin blocks previously revealed by C-banding. The NORs varied in size among the homologues of the three populations. These findings of the populations studied support the hypothesis that the stability of NORs in the A. albitarsis complex is characterized by the presence of clustered and conserved sites in a unique pair of chromosomes.

摘要

首次在巴西的白跗按蚊（Nyssorhynchus）广义种群体中描述了C带模式以及核仁组织区（NORs）的大小和位置。C带显示常染色体以及端着丝粒（X）和点状（Y）性染色体的着丝粒异染色质块大小存在差异。荧光原位杂交表明，NORs位于性染色体（XX/XY）的着丝粒周围区域，这与之前C带揭示的着丝粒组成型异染色质块的数量和位置一致。在三个群体的同源染色体中，NORs的大小各不相同。对所研究群体的这些发现支持了以下假设：白跗按蚊复合体中NORs的稳定性特征是在一对独特的染色体中存在成簇且保守的位点。