猪上皮绒毛膜胎盘的建立与子宫内膜T细胞募集有关。
Establishment of the porcine epitheliochorial placenta is associated with endometrial T-cell recruitment.
作者信息
Dimova Tanya, Mihaylova Anastassia, Spassova Petia, Georgieva Rayna
机构信息
Department of Immunobiology of Reproduction, Institute of Biology and Immunology of Reproduction, Acad.K.Bratanov, Bulgarian Academy of Sciences, Sofia, Bulgaria.
出版信息
Am J Reprod Immunol. 2007 Apr;57(4):250-61. doi: 10.1111/j.1600-0897.2007.00472.x.
PROBLEM
We assessed implantation-associated quantitative changes in peripheral blood and endometrial T lymphocytes throughout epitheliochorial placenta formation.
METHOD OF STUDY
T-cell subsets were investigated in 10-, 15-, 20-, 30-, and 40-day pregnant and non-pregnant sows by flow cytometry and immunohistochemistry.
RESULTS
Endometrial total T, T cytotoxic (Tc), and T helper (Th) cells were in peak numbers at the attachment phase of implantation and Tc cells persisted in high proportions up to placental establishment. The number of gammadelta T lymphocytes was relatively small and implantation-independent. In situ, T cells increased in number with the advancement of implantation and formed T-cell clusters with implantation phase-dependent location. Percentages of peripheral blood T cells were not significantly changed throughout the implantation.
CONCLUSION
Superficial and adeciduate implantation of pigs has a profound effect on the number of total T, Tc, and Th cells and pattern of distribution of endometrial T cells in situ.
问题
我们评估了在整个上皮绒毛膜胎盘形成过程中，外周血和子宫内膜T淋巴细胞与着床相关的定量变化。
研究方法
通过流式细胞术和免疫组织化学，对怀孕10天、15天、20天、30天和40天的母猪以及未怀孕母猪的T细胞亚群进行了研究。
结果
子宫内膜总T细胞、细胞毒性T细胞（Tc）和辅助性T细胞（Th）在着床附着期数量达到峰值，并且Tc细胞在胎盘形成前一直保持高比例。γδT淋巴细胞数量相对较少，且与着床无关。在原位，T细胞数量随着着床进程增加，并形成了与着床阶段相关位置的T细胞簇。在整个着床过程中，外周血T细胞百分比没有显著变化。
结论
猪的浅表性和非蜕膜性着床对子宫内膜总T细胞、Tc细胞和Th细胞数量以及原位子宫内膜T细胞的分布模式有深远影响。
Zotero 插件