Taneja R, Gopalkrishnan R, Gopinathan K P
Department of Microbiology, Indian Institute of Science, Bangalore.
Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1070-4. doi: 10.1073/pnas.89.3.1070.
The glycine tRNA genes in silkworm Bombyx mori contain two regulatory regions upstream of the transcription start site as identified by direct transcription of 5' deletion mutants, transcription competition, gel mobility shift assays, and footprinting. A positive regulatory region is present in the immediate 5' flanking sequences of the four tRNA 1Gly clones studied. This region is essential for cell-free transcription in homologous extracts. A negative regulatory region is present farther upstream, and transcription competition experiments indicate its presence in three of the four clones examined.
通过对5'缺失突变体进行直接转录、转录竞争、凝胶迁移率变动分析和足迹法鉴定，家蚕（Bombyx mori）中的甘氨酸tRNA基因在转录起始位点上游包含两个调控区域。在所研究的四个tRNA 1Gly克隆的紧邻5'侧翼序列中存在一个正调控区域。该区域对于同源提取物中的无细胞转录至关重要。一个负调控区域位于更上游，转录竞争实验表明在所检测的四个克隆中的三个中存在该区域。
