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家蚕甘氨酸转运RNA基因的结构与体外转录

Structure and in vitro transcription of a glycine tRNA gene from Bombyx mori.

作者信息

Fournier A, Guérin M A, Corlet J, Clarkson S G

出版信息

EMBO J. 1984 Jul;3(7):1547-52. doi: 10.1002/j.1460-2075.1984.tb02009.x.

DOI:10.1002/j.1460-2075.1984.tb02009.x
PMID:6745242
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557557/
Abstract

We report the sequences of a Bombyx mori tRNA1Gly gene, its flanking regions, and its in vitro transcription products. The 5' flanking DNA contains the sequences TATAC, TATTTT and TTC located 30, 18 and 4 nucleotides, respectively, in front of the transcription initiation site. These resemble, in both position and composition, sequences preceding other RNA polymerase III genes of B. mori. A deletion mutant retaining these conserved sequences and an additional 8 bp of flanking DNA is transcribed better than the wild-type gene in cell-free extracts from Xenopus laevis and B. mori. A mutant lacking the conserved sequences is expressed in the frog extract, but is inactive in the homologous system.

摘要

我们报道了家蚕tRNA1Gly基因的序列、其侧翼区域及其体外转录产物。5'侧翼DNA在转录起始位点前分别位于30、18和4个核苷酸处含有TATAC、TATTTT和TTC序列。这些序列在位置和组成上与家蚕其他RNA聚合酶III基因之前的序列相似。保留这些保守序列和额外8 bp侧翼DNA的缺失突变体在非洲爪蟾和家蚕的无细胞提取物中比野生型基因转录得更好。缺乏保守序列的突变体在青蛙提取物中表达，但在同源系统中无活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29b/557557/0b2a4f12055a/emboj00311-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29b/557557/377da37c75c8/emboj00311-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29b/557557/0b2a4f12055a/emboj00311-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29b/557557/377da37c75c8/emboj00311-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29b/557557/0b2a4f12055a/emboj00311-0104-a.jpg

相似文献

1
Structure and in vitro transcription of a glycine tRNA gene from Bombyx mori.家蚕甘氨酸转运RNA基因的结构与体外转录
EMBO J. 1984 Jul;3(7):1547-52. doi: 10.1002/j.1460-2075.1984.tb02009.x.
2
5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems.家蚕丙氨酸tRNA基因在同源体外转录系统中的活性需要5'侧翼序列信号。
Cell. 1980 Nov;22(1 Pt 1):171-8. doi: 10.1016/0092-8674(80)90165-8.
3
Regulation of glycine tRNA gene expression in the posterior silk glands of the silkworm Bombyx mori.家蚕后部丝腺中甘氨酸tRNA基因表达的调控
Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1070-4. doi: 10.1073/pnas.89.3.1070.
4
In vitro transcription of a silkworm 5S RNA gene requires an upstream signal.家蚕5S RNA基因的体外转录需要一个上游信号。
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5519-22. doi: 10.1073/pnas.81.17.5519.
5
Silkworm 5S RNA and alanine tRNA genes share highly conserved 5' flanking and coding sequences.家蚕5S核糖体RNA和丙氨酸转运RNA基因具有高度保守的5'侧翼序列和编码序列。
Mol Cell Biol. 1982 Dec;2(12):1524-31. doi: 10.1128/mcb.2.12.1524-1531.1982.
6
The primary transcription product of a silkworm alanine tRNA gene: identification of in vitro sites of initiation, termination and processing.家蚕丙氨酸tRNA基因的初级转录产物：体外起始、终止和加工位点的鉴定
Cell. 1979 Dec;18(4):1217-29. doi: 10.1016/0092-8674(79)90234-4.
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Role of TATATAA element in the regulation of tRNA1Gly gene expression in Bombyx mori is position dependent.TATATAA元件在家蚕tRNA1Gly基因表达调控中的作用具有位置依赖性。
J Mol Biol. 1996 Oct 4;262(4):396-406. doi: 10.1006/jmbi.1996.0523.
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Bombyx mori nuclear polyhedrosis virus-based vectors for expressing passenger genes in silkmoth cells under viral or cellular promoter control.基于家蚕核型多角体病毒的载体，用于在病毒或细胞启动子控制下在家蚕细胞中表达外源基因。
Gene. 1989 Jan 30;75(1):59-71. doi: 10.1016/0378-1119(89)90383-1.
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Transcription of a cloned Bombyx mori tRNA2Ala gene: nucleotide sequence of the tRNA precursor and its processing in vitro.克隆的家蚕tRNA2Ala基因的转录：tRNA前体的核苷酸序列及其体外加工
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Identification of precursor molecules to individual tRNA species from Bombyx mori.家蚕中各tRNA种类前体分子的鉴定。
Proc Natl Acad Sci U S A. 1978 Feb;75(2):635-9. doi: 10.1073/pnas.75.2.635.

引用本文的文献

1
Silkworm TFIIIB binds both constitutive and silk gland-specific tRNA Ala promoters but protects only the constitutive promoter from DNase I cleavage.家蚕TFIIIB能结合组成型和丝腺特异性tRNA丙氨酸启动子，但仅保护组成型启动子免受DNase I切割。
Mol Cell Biol. 1996 Mar;16(3):1256-66. doi: 10.1128/MCB.16.3.1256.
2
Transcription of a silkworm tRNA(cAla) gene is directed by two AT-rich upstream sequence elements.家蚕tRNA（cAla）基因的转录由两个富含AT的上游序列元件指导。
Nucleic Acids Res. 1993 Dec 25;21(25):5875-81. doi: 10.1093/nar/21.25.5875.
3
In vitro transcription of a silkworm 5S RNA gene requires an upstream signal.

本文引用的文献

1
A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.短的 5'侧翼区含有保守序列，这对于家蚕丝氨酸 tRNA 基因的活性是必需的。
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3416-20. doi: 10.1073/pnas.80.11.3416.
2
Silkworm 5S RNA and alanine tRNA genes share highly conserved 5' flanking and coding sequences.家蚕5S核糖体RNA和丙氨酸转运RNA基因具有高度保守的5'侧翼序列和编码序列。
Mol Cell Biol. 1982 Dec;2(12):1524-31. doi: 10.1128/mcb.2.12.1524-1531.1982.
3
5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems.
家蚕5S RNA基因的体外转录需要一个上游信号。
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5519-22. doi: 10.1073/pnas.81.17.5519.
4
Conserved sequences in both coding and 5' flanking regions of mammalian opal suppressor tRNA genes.哺乳动物乳白抑制tRNA基因编码区和5'侧翼区的保守序列。
Nucleic Acids Res. 1985 Jul 11;13(13):4765-75. doi: 10.1093/nar/13.13.4765.
5
Upstream sequences confer distinctive transcriptional properties on genes encoding silkgland-specific tRNAAla.上游序列赋予编码丝腺特异性丙氨酸转运RNA的基因独特的转录特性。
Proc Natl Acad Sci U S A. 1986 Jan;83(2):374-8. doi: 10.1073/pnas.83.2.374.
6
Sequence of a lysine tRNA gene from Bombyx mori.家蚕赖氨酸转运RNA基因序列
Nucleic Acids Res. 1986 Feb 25;14(4):1915. doi: 10.1093/nar/14.4.1915.
7
Sequence of a glutamic acid tRNA gene from Bombyx mori.家蚕谷氨酸转运RNA基因序列
Nucleic Acids Res. 1986 Feb 25;14(4):1916. doi: 10.1093/nar/14.4.1916.
8
Transcriptional properties of BmX, a moderately repetitive silkworm gene that is an RNA polymerase III template.BmX的转录特性，BmX是一种中等重复的家蚕基因，是RNA聚合酶III的模板。
Mol Cell Biol. 1988 Feb;8(2):624-31. doi: 10.1128/mcb.8.2.624-631.1988.
9
Compilation of tRNA sequences and sequences of tRNA genes.tRNA序列及tRNA基因序列的汇编。
Nucleic Acids Res. 1991 Apr 25;19 Suppl(Suppl):2127-71. doi: 10.1093/nar/19.suppl.2127.
10
Regulation of glycine tRNA gene expression in the posterior silk glands of the silkworm Bombyx mori.家蚕后部丝腺中甘氨酸tRNA基因表达的调控
Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1070-4. doi: 10.1073/pnas.89.3.1070.
家蚕丙氨酸tRNA基因在同源体外转录系统中的活性需要5'侧翼序列信号。
Cell. 1980 Nov;22(1 Pt 1):171-8. doi: 10.1016/0092-8674(80)90165-8.
4
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.5S RNA基因中心的一个控制区域指导转录的特异性起始：I. 该区域的5'边界。
Cell. 1980 Jan;19(1):13-25. doi: 10.1016/0092-8674(80)90384-0.
5
A split promoter for a eucaryotic tRNA gene.一种真核生物tRNA基因的分裂启动子。
Cell. 1981 May;24(2):573-85. doi: 10.1016/0092-8674(81)90348-2.
6
A walk in the chorion locus of Bombyx mori.家蚕绒毛膜位点漫步
Cell. 1982 Jun;29(2):633-43. doi: 10.1016/0092-8674(82)90179-9.
7
Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.酵母SUP4 tRNATyr基因座的突变：突变基因的体外转录
Cell. 1980 Nov;22(2 Pt 2):415-25. doi: 10.1016/0092-8674(80)90352-9.
8
Internal control regions for transcription of eukaryotic tRNA genes.真核生物tRNA基因转录的内部控制区域。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6657-61. doi: 10.1073/pnas.78.11.6657.
9
The extent of a eukaryotic tRNA gene. 5'- and 3'-flanking sequence dependence for transcription and stable complex formation.真核生物tRNA基因的范围。转录和稳定复合物形成对5'和3'侧翼序列的依赖性。
J Biol Chem. 1984 Feb 10;259(3):1461-7.
10
5'-flanking sequences that inhibit in vitro transcription of a xenopus laevis tRNA gene.抑制非洲爪蟾tRNA基因体外转录的5'侧翼序列。
Cell. 1983 Oct;34(3):881-90. doi: 10.1016/0092-8674(83)90545-7.