C6胶质瘤细胞系中SP/非SP表型的波动

Fluctuation of the SP/non-SP phenotype in the C6 glioma cell line.

作者信息

Platet Nadine, Mayol Jean-François, Berger François, Hérodin Francis, Wion Didier

机构信息

INSERM U318, CHU Michallon, 38043 Grenoble, France.

出版信息

FEBS Lett. 2007 Apr 3;581(7):1435-40. doi: 10.1016/j.febslet.2007.02.071. Epub 2007 Mar 7.

DOI:10.1016/j.febslet.2007.02.071

PMID:17362939

Abstract

Using the C6 glioma cell as a paradigm, we found that (i) the clonogenicity of C6 cells is several orders of magnitude higher than the percentage of SP cells; (ii) non-SP cells are able to generate SP cells, and conversely SP cells generate non-SP cells; (iii) non-SP sorted cells behave as tumorigenic cells. Hence, in C6 cells cultured in serum-containing medium, SP cells can be generated from non-SP cells. This dynamic equilibrium explains in C6 cells the maintenance of the SP phenotype with cell passaging and demonstrates the existence of tumorigenic non-SP cells.

摘要

以C6胶质瘤细胞作为范例，我们发现：（i）C6细胞的克隆形成能力比侧群（SP）细胞的百分比高几个数量级；（ii）非SP细胞能够产生SP细胞，反之亦然，SP细胞也能产生非SP细胞；（iii）分选得到的非SP细胞具有致瘤细胞的特性。因此，在含血清培养基中培养的C6细胞中，非SP细胞能够产生SP细胞。这种动态平衡解释了在C6细胞中SP表型随细胞传代得以维持的现象，并证明了具有致瘤性的非SP细胞的存在。