Roffey Ben W C, Atwal Avtar S, Johns Timothy, Kubow Stan
School of Dietetics and Human Nutrition, McGill University, Macdonald Campus, 21,111 Lakeshore, Ste-Anne-de-Bellevue, Que., Canada H9X 3V9.
J Ethnopharmacol. 2007 May 30;112(1):77-84. doi: 10.1016/j.jep.2007.02.003. Epub 2007 Feb 11.
To examine the effects of Momordica charantia on glucose uptake and adiponectin secretion in adipose cells, 3T3-L1 adipocytes were treated with three concentrations (0.2, 0.3 and 0.4mg/ml) of water and ethanol extracts of Momordica charantia fruit and seeds alone and in combination with either 0.5nM or 50nM insulin. The treatment combination of 0.2mg/ml water extract and 0.5nM insulin was associated with significant (p<0.05) increases in glucose uptake (61%) and adiponectin secretion (75%) over control levels. The ethanol extract was not associated with an increase in glucose uptake; however, a dose-dependent decrease in basal glucose uptake and insulin-mediated glucose uptake was observed with the ethanol extract in combination with 50nM insulin. In the absence of insulin, no effects on glucose uptake were observed in adipocytes exposed to the water extracts whereas the highest concentration (0.4mg/ml) of the ethanol extract was associated with a significant (p<0.05) decrease in glucose uptake relative to controls. The present results indicate that water-soluble component(s) in Momordica charantia enhance the glucose uptake at sub-optimal concentrations of insulin in 3T3-L1 adipocytes, which is accompanied by and may be a result of increased adiponectin secretion from the 3T3-L1 adipocytes.
为了研究苦瓜对脂肪细胞葡萄糖摄取和脂联素分泌的影响，将3T3-L1脂肪细胞分别用三种浓度（0.2、0.3和0.4mg/ml）的苦瓜果实和种子的水提取物及乙醇提取物单独处理，以及与0.5nM或50nM胰岛素联合处理。与对照水平相比，0.2mg/ml水提取物和0.5nM胰岛素的联合处理使葡萄糖摄取量显著增加（61%），脂联素分泌量显著增加（75%）（p<0.05）。乙醇提取物与葡萄糖摄取量增加无关；然而，乙醇提取物与50nM胰岛素联合处理时，基础葡萄糖摄取量和胰岛素介导的葡萄糖摄取量呈剂量依赖性降低。在无胰岛素的情况下，暴露于水提取物的脂肪细胞中未观察到对葡萄糖摄取的影响，而乙醇提取物的最高浓度（0.4mg/ml）与相对于对照的葡萄糖摄取量显著降低（p<0.05）有关。目前的结果表明，苦瓜中的水溶性成分在次优胰岛素浓度下可增强3T3-L1脂肪细胞的葡萄糖摄取，这伴随着3T3-L1脂肪细胞脂联素分泌增加，且可能是其结果。
