Wyckoff Jeffrey B, Wang Yarong, Lin Elaine Y, Li Jiu-feng, Goswami Sumanta, Stanley E Richard, Segall Jeffrey E, Pollard Jeffrey W, Condeelis John
Gruss Lipper Center for Biophotonics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Cancer Res. 2007 Mar 15;67(6):2649-56. doi: 10.1158/0008-5472.CAN-06-1823.
Although the presence of macrophages in tumors has been correlated with poor prognosis, until now there was no direct observation of how macrophages are involved in hematogenous metastasis. In this study, we use multiphoton microscopy to show, for the first time, that tumor cell intravasation occurs in association with perivascular macrophages in mammary tumors. Furthermore, we show that perivascular macrophages of the mammary tumor are associated with tumor cell intravasation in the absence of local angiogenesis. These results show that the interaction between macrophages and tumor cells lying in close proximity defines a microenvironment that is directly involved in the intravasation of cancer cells in mammary tumors.
尽管肿瘤中巨噬细胞的存在与预后不良相关，但直到现在还没有对巨噬细胞如何参与血行转移进行直接观察。在本研究中，我们首次使用多光子显微镜显示，乳腺肿瘤中的肿瘤细胞内渗与血管周围巨噬细胞有关。此外，我们还表明，在没有局部血管生成的情况下，乳腺肿瘤的血管周围巨噬细胞与肿瘤细胞内渗有关。这些结果表明，紧密相邻的巨噬细胞与肿瘤细胞之间的相互作用定义了一个直接参与乳腺肿瘤癌细胞内渗的微环境。
