Hulit James, Kedrin Dmitriy, Gligorijevic Bojana, Entenberg David, Wyckoff Jeffrey, Condeelis John, Segall Jeffrey E
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, USA.
Methods Mol Biol. 2012;872:15-30. doi: 10.1007/978-1-61779-797-2_2.
The analysis of cancer cell behavior in the primary tumor in living animals provides an opportunity to explore the process of invasion and intravasation in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-flap tumor dissection and a mammary imaging window.
对活体动物原发性肿瘤中癌细胞行为的分析为探索体内复杂微环境中的侵袭和血管内渗过程提供了契机。在本章中，我们描述了我们开发的用于对乳腺肿瘤进行活体成像的方法。我们提供了通过注射肿瘤细胞系生成肿瘤的程序，以及使用皮瓣肿瘤解剖和乳腺成像窗口进行多光子成像的程序。
