Thiel Vera, Leininger Sven, Schmaljohann Rolf, Brümmer Franz, Imhoff Johannes F
Leibniz-Institut für Meereswissenschaften, IFM-GEOMAR, Düsternbrooker Weg 20, 24105, Kiel, Germany.
Microb Ecol. 2007 Jul;54(1):101-11. doi: 10.1007/s00248-006-9177-y. Epub 2007 Mar 16.
A stable and specific bacterial community was shown to be associated with the Mediterranean sponge Chondrilla nucula. The associated bacterial communities were demonstrated to be highly similar for all studied specimens regardless of sampling time and geographical region. In addition, analysis of 16S rDNA clone libraries revealed three constantly C. nucula-associated bacterial phylotypes belonging to the Acidobacteria, the Gamma- and Deltaproteobacteria present in sponge specimens from two Mediterranean regions with distinct water masses (Ligurian Sea and Adriatic Sea). For the first time, candidate division TM7 bacteria were found in marine sponges. A major part (79%) of the C. nucula-derived 16S rDNA sequences were closely related to other sponge-associated bacteria. Phylogenetic analysis identified 14 16S rRNA gene sequence clusters, seven of which consisted of exclusively sponge-derived sequences, whereas the other seven clusters contained additional environmental sequences. This study adds to a growing database on the stability and variability of microbial consortia associated with marine sponges.
一个稳定且特定的细菌群落被证明与地中海海绵核果软海绵（Chondrilla nucula）相关联。对于所有研究的标本，无论采样时间和地理区域如何，相关的细菌群落都显示出高度相似性。此外，对16S rDNA克隆文库的分析揭示了三个始终与核果软海绵相关的细菌系统型，它们分别属于酸杆菌门、γ-变形菌纲和δ-变形菌纲，存在于来自两个具有不同水体的地中海区域（利古里亚海和亚得里亚海）的海绵标本中。首次在海洋海绵中发现了候选分类群TM7细菌。核果软海绵衍生的16S rDNA序列的大部分（79%）与其他与海绵相关的细菌密切相关。系统发育分析确定了14个16S rRNA基因序列簇，其中七个仅由海绵衍生的序列组成，而其他七个簇包含额外的环境序列。这项研究为与海洋海绵相关的微生物群落的稳定性和变异性不断增长的数据库增添了内容。
