Schulpis Kleopatra H, Lazaropoulou Christina, Vlachos George D, Partsinevelos George A, Michalakakou Kelly, Gavrili Stavroula, Gounaris Antonia, Antsaklis Aris, Papassotiriou Ioannis
Institute of Child Health, Research Center, Department of Clinical Biochemistry Aghia Sophia Children's Hospital, and First Department of Obstetrics and Gynecology, Athens University Medical School, Greece.
Acta Obstet Gynecol Scand. 2007;86(3):320-6. doi: 10.1080/00016340601181706.
To investigate the effect of the mode of labour and delivery on the total antioxidant status (TAS), and the biomarker of DNA oxidation, 8-hydroxy-deoxyguanosine (8-OHdG) serum levels, in mothers and their newborns.
Some 106 women with normal pregnancy and normal blood biochemical parameters were divided into 4 groups: Group A (n=28) with normal labour and vaginal delivery (VG), Group B (n=25) with scheduled cesarean section (CS), Group C (n=26) with 'emergency' CS, and Group D (n=27) with prolonged labour+VG. Blood was obtained from the mothers at the beginning of labour, and immediately after delivery (pre- and post-delivery), as well as from the umbilical cord (CB). TAS, 8-OHdG and creatine kinase (CK) were measured in the sera with appropriate methodology.
TAS levels were almost similar in all the groups pre-delivery, and in CB irrespective of the mode of labour and delivery, and remarkably decreased in Groups C and D post-delivery. 8-OHdG levels in Group C (0.94+/-0.08 ng/ml) and Group D (0.98+/-0.08 ng/ml) were significantly higher than those in Group A (0.26+/-0.01 ng/ml, p<0.001) and Group B (0.28+/-0.07 ng/ml, p<0.001) post-delivery. 8-OHdG levels were low in CB, independent of the mode of labour. CK positively correlated with 8-OHdG (r=0.48, p<0.001), the latter negatively correlated with TAS (r=-0.53, p<0.01).
The lowest TAS and the highest 8-OHdG levels were found in Groups C and D post-delivery, probably due to the long-term participation of the mothers' skeletal and uterus muscles, whereas 8-OHdG levels were low in CB irrespective of the mode of delivery, possibly as a consequence of the antioxidant action of the placenta and/or the low lipid levels in the serum of the umbilical cord.
研究分娩方式对母亲及其新生儿的总抗氧化状态（TAS）以及DNA氧化生物标志物8-羟基脱氧鸟苷（8-OHdG）血清水平的影响。
约106例妊娠正常且血液生化参数正常的女性被分为4组：A组（n = 28）为正常分娩且经阴道分娩（VG）；B组（n = 25）为择期剖宫产（CS）；C组（n = 26）为“急诊”剖宫产；D组（n = 27）为产程延长+经阴道分娩。在分娩开始时、分娩后即刻（分娩前和分娩后）采集母亲的血液，以及脐带血（CB）。采用适当方法测定血清中的TAS、8-OHdG和肌酸激酶（CK）。
分娩前所有组以及脐带血中的TAS水平几乎相似，且与分娩方式无关，而C组和D组在分娩后TAS水平显著下降。分娩后，C组（0.94±0.08 ng/ml）和D组（0.98±0.08 ng/ml）的8-OHdG水平显著高于A组（0.26±0.01 ng/ml，p<0.001）和B组（0.28±0.07 ng/ml，p<0.001）。脐带血中的8-OHdG水平较低，与分娩方式无关。CK与8-OHdG呈正相关（r = 0.48，p<0.001），后者与TAS呈负相关（r = -0.53，p<0.01）。
分娩后C组和D组的TAS水平最低，8-OHdG水平最高，这可能是由于母亲骨骼肌和子宫肌肉长期参与所致，而无论分娩方式如何，脐带血中的8-OHdG水平都较低，这可能是胎盘抗氧化作用和/或脐带血清中脂质水平较低的结果。
