Vlachos Dimitrios G, Schulpis Kleopatra H, Parthimos Theodore, Mesogitis Spyros, Vlachos George D, Partsinevelos George A, Antsaklis Aris, Tsakiris Stylianos
Department of Physiology, Medical School, Athens University, P.O. Box 65257, Athens 15401, Greece.
Clin Biochem. 2008 Jul;41(10-11):818-23. doi: 10.1016/j.clinbiochem.2008.04.005. Epub 2008 Apr 18.
Free radical production and high catecholamine levels are implicated with the modulation of acetylcholinesterase (AChE) activity.
To investigate the effect of the mode of delivery on maternal-neonatal erythrocyte membrane AChE activity.
Some women with normal pregnancy (N = 30) were divided into two groups: group A (N = 16) with normal labour and vaginal delivery and group B (N = 14) with scheduled Cesarean section, twenty non-pregnant women were the controls. Blood was obtained from controls and from mothers pre- vs post-delivery as well as from the umbilical cord (CB). Total antioxidant status (TAS), membrane AChE activities and catecholamine blood levels were measured with a commercial kit, spectrophotometrically and HPLC methods, respectively.
TAS and catecholamine levels as well as membrane AChE activities were similar in the two groups of mothers pre-delivery and in controls. TAS levels were reduced whereas AChE activities and catecholamine levels were increased post-delivery in mothers of group A and unaltered in group B at the same times of study. AChE activity was similarly lower in the CB of neonates than those of their mothers pre-delivery.
During a normal delivery process, the low TAS, the increased levels of catecholamines and the increased AChE activity, post-delivery, may be due to the increased stress due to the participation of uterus and skeletal muscles as during endurance exercise. The low AChE activity in newborns may be related to perinatal immaturity.
自由基生成和高儿茶酚胺水平与乙酰胆碱酯酶（AChE）活性的调节有关。
研究分娩方式对母婴红细胞膜AChE活性的影响。
将一些正常妊娠的妇女（N = 30）分为两组：A组（N = 16）经正常产程阴道分娩，B组（N = 14）行择期剖宫产，20名未孕妇女作为对照组。分别从对照组、母亲分娩前后以及脐带（CB）采集血液。采用商用试剂盒、分光光度法和高效液相色谱法分别测定总抗氧化状态（TAS）、膜AChE活性和血液中儿茶酚胺水平。
两组母亲分娩前及对照组的TAS、儿茶酚胺水平以及膜AChE活性相似。在相同研究时间，A组母亲分娩后TAS水平降低，而AChE活性和儿茶酚胺水平升高，B组则无变化。新生儿脐带血中的AChE活性同样低于其母亲分娩前。
在正常分娩过程中，分娩后TAS降低、儿茶酚胺水平升高以及AChE活性增加，可能是由于子宫和骨骼肌参与导致的应激增加，类似于耐力运动时的情况。新生儿AChE活性低可能与围产期不成熟有关。
