Tsuzuki Yoko, Yamashita Yoriko, Hattori Yuka, Hua Li Guang, Akatsuka Shinya, Kotani Tomomi, Kikkawa Fumitaka, Naiki-Ito Aya, Takahashi Satoru, Nishiwaki Kimitoshi, Toyokuni Shinya
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan; Department of Anesthesiology and Resuscitation, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan; Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan; Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
J Clin Biochem Nutr. 2016 Mar;58(2):156-60. doi: 10.3164/jcbn.15-138. Epub 2016 Feb 17.
Anesthesia is sometimes used for the reduction of maternal pain in normal human term labor, but whether the drugs affect oxidative stress remains unclear. The placenta serves as an interface between the maternal and fetal vasculature. In this study, we immunohistochemically analyzed two markers for oxidative stress, namely 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 4-hydroxy-2-nonenal-modified proteins (HNE), using placentas from 21 cases of normal tansvaginal delivery (V group), 20 Caesarean sections (C group), and 17 normal transvaginal deliveries with epidural anesthesia (E group). 8-OHdG staining in the nuclei of trophoblasts lining the chorionic villi was significantly stronger in the V group either compared with the C or E group (p<0.001), without significant differences in the C and E groups (p = 0.792). Moderate to intense staining by HNE of the intravascular serum of chorionic villi vasculature was frequently observed in the placentas from the V group, but less frequently of those in either C or E groups (p<0.001), nor the p value comparing the C and E groups was significant (p = 0.128) for HNE staining. Our results suggest that although the role of oxidative stress and its influences on fetal state in the placenta in labor remains unclear, it seems to be lessened by epidural anesthesia.
在正常足月分娩时，有时会使用麻醉来减轻产妇疼痛，但这些药物是否会影响氧化应激仍不清楚。胎盘是母体和胎儿血管系统之间的界面。在本研究中，我们使用来自21例正常经阴道分娩（V组）、20例剖宫产（C组）和17例硬膜外麻醉下正常经阴道分娩（E组）的胎盘，通过免疫组织化学分析了两种氧化应激标志物，即8-羟基-2'-脱氧鸟苷（8-OHdG）和4-羟基-2-壬烯醛修饰蛋白（HNE）。与C组或E组相比，V组中绒毛膜绒毛内衬滋养层细胞核中的8-OHdG染色明显更强（p<0.001），C组和E组之间无显著差异（p = 0.792）。在V组胎盘的绒毛血管系统血管内血清中经常观察到HNE的中度至强烈染色，但在C组或E组中较少见（p<0.001），C组和E组之间HNE染色的p值也无显著性差异（p = 0.128）。我们的结果表明，虽然分娩时胎盘中氧化应激的作用及其对胎儿状态的影响尚不清楚，但硬膜外麻醉似乎会减轻这种影响。
