Rho Young Soo, Lim Young Chang, Park Il Seok, Kim Jin Hwan, Ahn Hwoe Young, Cho Seong Jin, Shin Hyung Sik
Department of Otorhinolaryngology, Head and Neck Surgery, College of Medicine, Hallym University, Seoul, South Korea.
Acta Otolaryngol. 2007 Jan;127(1):76-81. doi: 10.1080/00016480600740571.
We conclude that increased expression level of the high mobility group I (HMGI(Y)) is closely associated with malignant transformation in head and neck squamous cell carcinomas (HNSCCs), and the measurement of HMGI(Y) levels in HNSCCs may be useful as a prognostic marker.
To investigate whether HMGI overexpression is observed in HNSCCs, and its value as a prognostic marker in HNSCCs.
HMGI(Y) expression was determined at the protein level by immunohistochemisty using a HMGI(Y)-specific antibody and RT-PCR in 10 surgically resected specimens of non-neoplastic tissue (normal palatal tissue) and 40 HNSCCs. We also evaluated the association of HMGI(Y) overexpression within clinicopathologic parameters, i.e. clinical stage, pathologic grade, status of cervical lymph node metastasis, recurrence rate.
Expression of HMGI(Y) by immunohistochemical staining was observed in 35 of 40 (87.5%) HNSCC samples, whereas normal mucosa and/or the mucosa adjacent to the tumor tissue showed negative or weakly positive staining (p<0.05). Semi-quantification of HMGI(Y) by RT-PCR was 2.98+/-2.24 in cancer and 0.47+/-0.25 in normal tissue (p<0.001). High expression of HMGI(Y) was observed in recurrent cases, compared with non-recurrent cases (p<0.05). However, no significant correlation was observed between the levels of HMGI(Y) expression and other clinical factors such as clinical stage, pathologic grade, and status of cervical lymph node metastasis.
我们得出结论，高迁移率族蛋白I（HMGI(Y)）表达水平升高与头颈部鳞状细胞癌（HNSCC）的恶性转化密切相关，检测HNSCC中HMGI(Y)水平可能作为一种预后标志物。
研究HMGI在HNSCC中是否存在过表达及其作为HNSCC预后标志物的价值。
采用HMGI(Y)特异性抗体，通过免疫组织化学在蛋白质水平检测10例手术切除的非肿瘤组织（正常腭组织）和40例HNSCC标本中的HMGI(Y)表达，并进行逆转录聚合酶链反应（RT-PCR）。我们还评估了HMGI(Y)过表达与临床病理参数（即临床分期、病理分级、颈部淋巴结转移状态、复发率）之间的关联。
40例HNSCC样本中有35例（87.5%）通过免疫组织化学染色观察到HMGI(Y)表达，而正常黏膜和/或肿瘤组织邻近的黏膜呈阴性或弱阳性染色（p<0.05）。通过RT-PCR对HMGI(Y)进行半定量分析，癌组织中为2.98±2.24，正常组织中为0.47±0.25（p<0.001）。与未复发病例相比，复发病例中观察到HMGI(Y)高表达（p<0.05）。然而，HMGI(Y)表达水平与其他临床因素如临床分期、病理分级和颈部淋巴结转移状态之间未观察到显著相关性。
