Nam E S, Kim D H, Cho S J, Chae S W, Kim H Y, Kim S M, Han J J, Shin H S, Park Y E
Department of Pathology, Hallym University, Seoul, Korea.
Histopathology. 2003 May;42(5):466-71. doi: 10.1046/j.1365-2559.2003.01618.x.
We evaluated the association between HMGI(Y) expression and the detection of malignant cells by simple reverse transcriptase-polymerase chain reaction (RT-PCR) and correlated the level of HMGI(Y) expression and the clinicopathological data in gastric cancer.
We analysed HMGI(Y) expression in 62 gastric cancer tissues and 28 normal gastric tissues by RT-PCR and immunohistochemical study. HMGI(Y) expression evidenced by RT-PCR was observed in 42 (67.7%) of 62 gastric cancer samples, whereas eight (28.6%) of 28 normal gastric tissues were positive (P = 0.001). In immunohistochemical staining for HMGI(Y), 48 (77.4%) of 62 gastric cancers were positive for HMGI(Y), whereas four (6.5%) of 62 normal gastric mucosae around the tumour were weakly positive, particularly in cells of some hyperplastic glands (P < 0.001). There was no significant correlation between the levels of HMGI(Y) expression and stage, tumour size, histological grade, invasion depth, or lymph node metastasis.
Our results suggest that HMGI(Y) expression could be associated with malignant phenotype in human gastric tissue, and that HMGI(Y) may contribute to gastric tumorigenesis.
我们通过简单逆转录聚合酶链反应（RT-PCR）评估了HMGI(Y)表达与恶性细胞检测之间的关联，并将HMGI(Y)表达水平与胃癌的临床病理数据相关联。
我们通过RT-PCR和免疫组织化学研究分析了62例胃癌组织和28例正常胃组织中的HMGI(Y)表达。在62例胃癌样本中，42例（67.7%）通过RT-PCR检测到HMGI(Y)表达，而28例正常胃组织中有8例（28.6%）呈阳性（P = 0.001）。在HMGI(Y)的免疫组织化学染色中，62例胃癌中有48例（77.4%）HMGI(Y)呈阳性，而肿瘤周围62例正常胃黏膜中有4例（6.5%）呈弱阳性，特别是在一些增生腺体的细胞中（P < 0.001）。HMGI(Y)表达水平与分期、肿瘤大小、组织学分级、浸润深度或淋巴结转移之间无显著相关性。
我们的结果表明，HMGI(Y)表达可能与人胃组织中的恶性表型相关，并且HMGI(Y)可能参与胃癌发生。
