Kim D H, Park Y S, Park C J, Son K C, Nam E S, Shin H S, Ryu J W, Kim D S, Park C K, Park Y E
Department of Pathology, Hallym University, Seoul, Korea.
Int J Cancer. 1999 Aug 20;84(4):376-80. doi: 10.1002/(sici)1097-0215(19990820)84:4<376::aid-ijc8>3.0.co;2-n.
Expression of HMGI(Y), a nucleoprotein that binds to A/T rich sequences in the minor groove of the DNA helix, is observable in neoplastically transformed cells but not in normal cells. We have analyzed HMGI(Y) expression in colorectal cancer and evaluated its clinicopathologic significance. HMGI(Y) mRNA was measured by CRT-PCR (competitive reverse transcription-polymerase chain reaction). Immunohistochemical staining for HMGI(Y), p53 and Ki-67 was performed in the same colon cancer tissues, and the results in colorectal tissues were similar to those of RT-PCR. HMGI(Y) expression evidenced by RT-PCR was observed in 63 of 64 (98.4%) colorectal cancer samples, and 2 of 5 (40%) adenomatous polyps, whereas 21 normal colon samples were negative (p<0.001). High HMGI(Y) expression using CRT-PCR was found in colon cancers with a high Ki-67 labeling index (p<0.001). There was no significant correlation between the levels of HMGI(Y) expression and stage, tumor size, lymph node metastasis, histologic grade and immunohistochemical status of p53. Our results indicate that the HMGI(Y) expression may occur at an early stage of carcinogenesis and correlate with cell proliferation. Int. J. Cancer (Pred. Oncol.), 84:376-380, 1999.
HMGI(Y)是一种与DNA螺旋小沟中富含A/T序列结合的核蛋白，在肿瘤转化细胞中可观察到其表达，而在正常细胞中则无表达。我们分析了HMGI(Y)在结直肠癌中的表达，并评估了其临床病理意义。通过CRT-PCR（竞争性逆转录-聚合酶链反应）检测HMGI(Y) mRNA。对同一结肠癌组织进行HMGI(Y)、p53和Ki-67的免疫组织化学染色，结直肠组织中的结果与RT-PCR结果相似。RT-PCR检测显示，64例结直肠癌样本中有63例（98.4%）、5例腺瘤性息肉中有2例（40%）存在HMGI(Y)表达，而21例正常结肠样本均为阴性（p<0.001）。CRT-PCR检测发现，Ki-67标记指数高的结肠癌中HMGI(Y)表达也高（p<0.001）。HMGI(Y)表达水平与分期、肿瘤大小、淋巴结转移、组织学分级及p53免疫组织化学状态之间无显著相关性。我们的结果表明，HMGI(Y)表达可能发生在癌变早期，并与细胞增殖相关。《国际癌症杂志（肿瘤预测）》，84:376 - 380，1999年。
