重组血红蛋白作为人工氧载体。
Recombinant hemoglobins as artificial oxygen carriers.
作者信息
Fronticelli Clara, Koehler Raymond C, Brinigar William S
机构信息
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
出版信息
Artif Cells Blood Substit Immobil Biotechnol. 2007;35(1):45-52. doi: 10.1080/10731190600974467.
Abstract
This paper describes the approaches we have taken to construct a) mutant hemoglobins with different oxygen affinities, and b) mutant hemoglobins and myoglobins that polymerize to high molecular weight aggregates in an effort to prevent extravasation and the associated vasoactivity. In vivo testing indicates that exchange transfusion of polymeric hemoglobins in mice does not result in vasoactivity and that polymeric hemoglobins are effective oxygen carriers to ischemic tissues irrespective of their oxygen affinity and cooperativity.
摘要
本文描述了我们为构建以下物质所采用的方法
a）具有不同氧亲和力的突变血红蛋白，以及b）聚合成高分子量聚集体的突变血红蛋白和肌红蛋白，以防止血管外渗及相关的血管活性。体内测试表明，在小鼠中进行聚合血红蛋白的换血不会导致血管活性，并且聚合血红蛋白是缺血组织的有效氧载体，无论其氧亲和力和协同性如何。
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本文引用的文献
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Vascular response to infusions of a nonextravasating hemoglobin polymer.血管对非渗出性血红蛋白聚合物输注的反应。
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