BY-2细胞中响应激发素的类凝集素受体激酶基因

Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells.

作者信息

Sasabe Michiko, Naito Kana, Suenaga Hiroko, Ikeda Takako, Toyoda Kazuhiro, Inagaki Yoshishige, Shiraishi Tomonori, Ichinose Yuki

机构信息

Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan.

出版信息

DNA Seq. 2007 Apr;18(2):152-9. doi: 10.1080/10425170601060905.

DOI:10.1080/10425170601060905

PMID:17364827

Abstract

The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.

摘要

蛋白激酶抑制剂K252a和星形孢菌素对激发子诱导的植物防御反应的抑制作用表明，防御反应需要蛋白质磷酸化。我们通过差异显示法在烟草亮黄（BY-2）细胞中分离出一个编码烟草凝集素样受体蛋白激酶1（NtlecRK1）的cDNA克隆，该基因是一个激发子响应基因。NtlecRK在烟草中形成一个至少有三个成员的基因家族。所有三个NtlecRK基因都可能编码N端豆科凝集素结构域、跨膜结构域和C端丝氨酸/苏氨酸型蛋白激酶结构域。绿色荧光蛋白（GFP）融合显示NtlecRK1蛋白位于质膜上。此外，NtlecRK1和3对INF1激发素和细菌激发子harpin有反应。这些结果表明，NtlecRKs是位于膜上的蛋白激酶，在BY-2细胞的防御反应中被诱导。