Tabopda Turibio Kuiate, Liu Jiawei, Ngadjui Bonaventure Tchaleu, Luu Bang
Laboratoire de Chimie Organique des Substances Naturelles, LC3-UMR 7177 CNRS - Université Louis Pasteur, Centre de Neurochimie, Strasbourg, France.
Planta Med. 2007 Apr;73(4):376-80. doi: 10.1055/s-2007-967132. Epub 2007 Mar 15.
Activity-guided fractionation of root and leaf extracts from Elephantopus mollis led to the isolation of a new triterpene (1) and a new sesquiterpene lactone (2) together with five known sesquiterpene lactones (3-7). The structures of compounds 1 and 2 were determined based on their spectroscopic data. The cytotoxic activity of the isolated compounds was evaluated against neuroblastoma B104 cells. The sesquiterpene lactone-type compounds 2-7 were highly cytotoxic. Among these, compound (5) was the most cytotoxic and induced apoptosis of neuroblastoma B104 cells in a dose- and time-dependent manner. No significant difference was observed for cytotoxicity of compound 5 towards all 3 cell lines tested.
对地胆草的根和叶提取物进行活性导向分级分离，得到了一种新的三萜（1）和一种新的倍半萜内酯（2），以及五种已知的倍半萜内酯（3 - 7）。化合物1和2的结构通过光谱数据确定。评估了分离得到的化合物对神经母细胞瘤B104细胞的细胞毒性活性。倍半萜内酯类化合物2 - 7具有高度细胞毒性。其中，化合物（5）细胞毒性最强，并以剂量和时间依赖性方式诱导神经母细胞瘤B104细胞凋亡。化合物5对所有测试 的3种细胞系的细胞毒性未观察到显著差异。
