Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar-608002, Tamil Nadu, India.
Life Sci. 2010 Jan 30;86(5-6):178-84. doi: 10.1016/j.lfs.2009.11.021. Epub 2009 Dec 1.
Membrane bound adenosine triphosphatases (ATPases) and lysosomal enzymes play an important role in the pathology of myocardial infarction. This study was aimed to evaluate the combined preventive effects of quercetin and alpha-tocopherol on membrane bound ATPases and lysosomal enzymes in isoproterenol induced myocardial infarcted rats.
Male Wistar rats were pretreated with a combination of quercetin (10mg/kg) and alpha-tocopherol (10mg/kg) daily for 14 days. After the pretreatment period, isoproterenol (100mg/kg) was injected to rats at an interval of 24h for two days to induce myocardial infarction. The activities of ATPases and lysosomal enzymes were assayed.
Isoproterenol treated rats showed decreased levels of heart creatine kinase and lactate dehydrogenase. The activity of sodium potassium adenosine triphosphatase was decreased and the activities of magnesium adenosine triphosphatase and calcium adenosine triphosphatase were increased in isoproterenol treated rats. Also, the activities of beta-glucuronidase, beta-N-acetylglucosaminidase, beta-galactosidase, cathepsin-B and D were increased (serum and heart), but the activities of beta-glucuronidase and cathepsin-D were decreased in lysosomal fraction and increased in cytosolic fraction of the heart in isoproterenol treated rats. Furthermore, the heart lipid peroxidation products were increased in isoproterenol treated rats. Combined pretreatment with quercetin and alpha-tocopherol to isoproterenol treated rats normalized all the biochemical parameters studied. The observed effects are due to their membrane stabilizing property and this property might be due to decreased lipid peroxidation.
Our study demonstrated that combined pretreatment was better than single pretreatment. This study may have significant impact on myocardial infarcted patients.
细胞膜结合三磷酸腺苷酶（ATP 酶）和溶酶体酶在心肌梗死的病理过程中起着重要作用。本研究旨在评估槲皮素和α-生育酚联合预防对异丙肾上腺素诱导的心肌梗死大鼠细胞膜结合 ATP 酶和溶酶体酶的影响。
雄性 Wistar 大鼠每天用槲皮素（10mg/kg）和α-生育酚（10mg/kg）预处理 14 天。预处理期结束后，异丙肾上腺素（100mg/kg）每隔 24 小时给大鼠注射两次，以诱导心肌梗死。测定 ATP 酶和溶酶体酶的活性。
异丙肾上腺素处理的大鼠心脏肌酸激酶和乳酸脱氢酶水平降低。钠钾三磷酸腺苷酶的活性降低，镁三磷酸腺苷酶和钙三磷酸腺苷酶的活性增加。此外，β-葡糖苷酸酶、β-N-乙酰氨基葡萄糖苷酶、β-半乳糖苷酶、组织蛋白酶-B 和 D 的活性（血清和心脏）增加，但β-葡糖苷酸酶和组织蛋白酶-D 的活性在溶酶体部分减少，在异丙肾上腺素处理的大鼠心脏胞质部分增加。此外，异丙肾上腺素处理的大鼠心脏脂质过氧化产物增加。槲皮素和α-生育酚联合预处理可使异丙肾上腺素处理的大鼠所有生化参数正常化。观察到的效果是由于它们的膜稳定特性，这种特性可能是由于脂质过氧化减少所致。
我们的研究表明，联合预处理优于单一预处理。这项研究可能对心肌梗死患者有重要影响。
