阿拉米辛孔在二肉豆蔻酰磷脂酰胆碱双层膜中的相互作用。
Interaction of alamethicin pores in DMPC bilayers.
作者信息
Constantin D, Brotons G, Jarre A, Li C, Salditt T
机构信息
Institut für Röntgenphysik, Göttingen, Germany.
出版信息
Biophys J. 2007 Jun 1;92(11):3978-87. doi: 10.1529/biophysj.106.101204. Epub 2007 Mar 16.
Abstract
We have investigated the x-ray scattering signal of highly aligned multilayers of the zwitterionic lipid 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine containing pores formed by the antimicrobial peptide alamethicin as a function of the peptide/lipid ratio. We are able to obtain information on the structure factor of the pore fluid, which then yields the interaction potential between pores in the plane of the bilayers. Aside from a hard core with a radius corresponding to the geometric radius of the pore, we find a repulsive lipid-mediated interaction with a range of approximately 30 A and a contact value of 2.4 k(B)T. This result is in qualitative agreement with recent theoretical models.
摘要
我们研究了两性离子脂质1,2 - 二肉豆蔻酰 - sn - 甘油 - 3 - 磷脂酰胆碱高度排列的多层膜的X射线散射信号，该多层膜含有由抗菌肽阿拉米辛形成的孔，作为肽/脂质比的函数。我们能够获得关于孔流体结构因子的信息，进而得出双层平面中孔之间的相互作用势。除了具有与孔的几何半径相对应的半径的硬核外，我们发现了一种排斥性的脂质介导相互作用，其范围约为30 Å，接触值为2.4 k(B)T。这一结果与最近的理论模型在定性上一致。
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