多重单核苷酸多态性鉴别
Multiplex SNP discrimination.
作者信息
Fish Daniel J, Horne M Todd, Searles Robert P, Brewood Greg P, Benight Albert S
出版信息
Biophys J. 2007 May 15;92(10):L89-91. doi: 10.1529/biophysj.107.105320. Epub 2007 Mar 16.
Abstract
Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.
摘要
在DNA微阵列上研究了完全匹配双链体和含有单个碱基对错配（单核苷酸多态性，SNPs）的双链体的多重杂交反应。确定了双链体长度、G-C百分比以及SNP的相对位置对双链体杂交和SNP分辨率的影响。我们的多重杂交理论模型准确地预测了观察结果，并表明靶标浓度是多重SNP检测中的一个关键变量。
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