Chagovetz Alexander, Blair Steve
Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT 84112, USA.
Biochem Soc Trans. 2009 Apr;37(Pt 2):471-5. doi: 10.1042/BST0370471.
DNA microarrays are plagued with inconsistent quantifications and false-positive results. Using established mechanisms of surface reactions, we argue that these problems are inherent to the current technology. In particular, the problem of multiplex non-equilibrium reactions cannot be resolved within the framework of the existing paradigm. We discuss the advantages and limitations of changing the paradigm to real-time data acquisition similar to real-time PCR methodology. Our analysis suggests that the fundamental problem of multiplex reactions is not resolved by the real-time approach itself. However, by introducing new detection chemistries and analysis approaches, it is possible to extract target-specific quantitative information from real-time microarray data. The possible scope of applications for real-time microarrays is discussed.
DNA微阵列存在定量不一致和假阳性结果的问题。利用已确立的表面反应机制，我们认为这些问题是当前技术所固有的。特别是，多重非平衡反应的问题无法在现有范式的框架内得到解决。我们讨论了将范式转变为类似于实时PCR方法的实时数据采集的优缺点。我们的分析表明，实时方法本身并不能解决多重反应的根本问题。然而，通过引入新的检测化学方法和分析方法，有可能从实时微阵列数据中提取目标特异性定量信息。本文还讨论了实时微阵列可能的应用范围。
