多重杂交动力学：机制与影响
Kinetics of multiplex hybridization: mechanisms and implications.
作者信息
Bishop J, Chagovetz A M, Blair S
机构信息
Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah, USA.
出版信息
Biophys J. 2008 Mar 1;94(5):1726-34. doi: 10.1529/biophysj.107.121459. Epub 2007 Nov 9.
Abstract
Quantitative analysis of DNA microarray data is complicated by uncertainties inherent to the experimental setup. Using computer simulations and real-time experimental results, we have previously demonstrated effects of multiplex reactions on a single sensing zone of an array, which may be a leading factor in erroneous interpretation of experimental data. We suggest here that a simplified three-component kinetic model may present a sufficient approximation to describe the general case of DNA sensing in a complex sample milieu. We show that, by analyzing the real-time hybridization kinetics of a nontarget species, we can perform quantitative analysis of unlabeled targets of interest within a broad dynamic range of concentrations.
摘要
DNA微阵列数据的定量分析因实验设置中固有的不确定性而变得复杂。利用计算机模拟和实时实验结果，我们先前已经证明了多重反应对阵列单个传感区域的影响，这可能是实验数据错误解读的一个主要因素。我们在此提出，一个简化的三组分动力学模型可能足以近似描述复杂样品环境中DNA传感的一般情况。我们表明，通过分析非目标物种的实时杂交动力学，我们可以在很宽的浓度动态范围内对感兴趣的未标记目标进行定量分析。
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