Pittner R A, Fain J N
Department of Biochemistry, University of Tennessee, Memphis.
Biochim Biophys Acta. 1992 Feb 3;1133(3):316-20. doi: 10.1016/0167-4889(92)90053-e.
Addition of ethanol (17 to 340 mM) to cultured rat hepatocytes stimulated the breakdown of phosphatidylcholine phospholipases D and C as measured by an increase in the rate of release of choline and phosphocholine into the medium. The effects of ethanol were mimicked by propanol, dimethylsulfoxide and to a lesser extent methanol. The magnitude of the stimulation seen with ethanol was equivalent to and additive to that produced by glucagon vasopressin, norepinephrine, A23187 or PMA. In contrast, ethanol (340 mM) stimulated PI-specific phospholipase C activity by less than 20%. An equivalent stimulation of PC-specific phospholipase D and C was seen with as little as 20 mM ethanol and a 100% increase was seen with 340 mM ethanol. Ethanol did not significantly affect the ability of vasopressin, norepinephrine, ATP or A23187 to stimulate PI-specific phospholipase C. It is concluded that while ethanol is only a weak stimulator of PI-specific phospholipase C, it is a potent stimulator of phosphatidylcholine breakdown in rat hepatocytes.
向培养的大鼠肝细胞中添加乙醇（17至340 mM），可刺激磷脂酰胆碱磷脂酶D和C的分解，这可通过培养基中胆碱和磷酸胆碱释放速率的增加来衡量。丙醇、二甲基亚砜以及程度较轻的甲醇可模拟乙醇的作用。乙醇产生的刺激程度与胰高血糖素、血管加压素、去甲肾上腺素、A23187或佛波酯（PMA）产生的刺激程度相当且具有加和性。相比之下，乙醇（340 mM）对磷脂酰肌醇特异性磷脂酶C活性的刺激小于20%。低至20 mM乙醇即可对磷脂酰胆碱特异性磷脂酶D和C产生同等程度的刺激，340 mM乙醇可使其增加100%。乙醇对血管加压素、去甲肾上腺素、ATP或A23187刺激磷脂酰肌醇特异性磷脂酶C的能力无显著影响。结论是，虽然乙醇只是磷脂酰肌醇特异性磷脂酶C的弱刺激剂，但它是大鼠肝细胞中磷脂酰胆碱分解的有效刺激剂。
