Vyas Neetu, Dua K K, Prakash Soam
Advanced and Environmental Parasitology Laboratory, Department of Zoology, Dayalbagh Educational Institute, Agra, India.
J Commun Dis. 2006 Mar;38(2):176-80.
This study was conducted in laboratory to evaluate the efficacy of filtered extracellular metabolites of Lagenidium giganteum against all the four instars of An. stephensi larvae. Fungal colonies have been cultured in PYG broth and after 15 days of culturing the fungus, metabolites have been filtered twice by whatman filter paper. These metabolites were again filtered by column chromatography and by rang syringe filters. Filtered metabolites were then used against all instars of An. stephensi larvae. The bioassays were conducted at five significantly different concentrations (1.68, 1.99, 2.17, 2.30, 2.40 ppm). The results suggest significant mortality on first three instar larvae and very low on fourth instar larvae.
本研究在实验室进行，以评估巨大拉格水霉过滤后的细胞外代谢产物对斯氏按蚊幼虫所有四个龄期的功效。真菌菌落已在PYG肉汤中培养，培养真菌15天后，代谢产物用沃特曼滤纸过滤两次。这些代谢产物再通过柱色谱法和针头式过滤器过滤。然后将过滤后的代谢产物用于斯氏按蚊幼虫的所有龄期。生物测定在五个显著不同的浓度（1.68、1.99、2.17、2.30、2.40 ppm）下进行。结果表明，前三龄幼虫死亡率显著，而四龄幼虫死亡率很低。
